Evaluation of Phenol Metabolic Enzymes in different Mustard (Brassica juncea L.) Cultivars Against Alternaria Blight (Alternaria brassicicola)

Abstract

The present study reports the variable antioxidant profile resulting from inducers / elicitors on mustard varieties against Alternaria brassicicola. An experiment was conducted with four inducers / elicitors viz., Benzothiadiazole, Hydrogen peroxide, Jasmonic Acid and Salicylic acid at three different concentrations to evaluate their effect against Alternaria leaf blight of mustard in four varieties viz., TBM-204, Bullet, B-54 and B-9. The pathogen A. brassicicola was inoculated at 15 DAS. An attempt was made to study the underline biochemical changes which may have their influence on induced resistance. Phenols secondary metabolites with responsible key enzymes of metabolism including phenylalanine ammonia lyase (PAL), Polyphenol oxidase (PPO), peroxidase (POD) were found to be more prevalent in the plants treated with BTH followed by Jasmonic acid, Salicylic acid and Hydrogen peroxide. Among the varieties, these enzymes were more prevalent in TBM-204 and Bullet and less prevalent in B-54 and B-9.