Evaluation of Apoptotic Activity of Ethanolic Extract of Andrographis paniculata Linn in Human Hepatocellular Carcinoma Cell Lines

Abstract

Introduction: Andrographis paniculata is one of the highly used potential medicinal plants in the world. This plant is traditionally used for the treatment of common cold, diarrhoea, fever due to several infective cause, jaundice, as a health tonic for the liver and cardiovascular health, and as an antioxidant. It is also used to improve sexual dysfunctions and serve as a contraceptive. All parts of this plant are used to extract the active phytochemicals, but the compositions of phytoconstituents widely differ from one part to another and with place, season, and time of harvest. Materials and Methods: All data obtained were analysed by Student t test using MS- Excel. The results were computed statistically using one way ANOVA.The reagents used were MTT [3-(4,5-dimethythiazol-2-yl) 2,5-diphenyl tetrazolium bromide]: 0.5 mg MTT/ml of serum-free DMEM, Solubilization solution: Dimethyl sulfoxide (DMSO)and Phosphate Buffered Saline (PBS; pH 7.4).The cytotoxic effects of lycopene Ag NP on 3T3 cells. Cells were treated with Lycopene Ag NP(10, 20, 30, 50, 100 and 200 μM) for 24 h, and cell viability was evaluated by MTT assay. Data are shown as means ± SD (n = 3). * compared with the control-blank group, p < 0.001. Results: The results were evaluated using three tests namely, MTT assay, Dual staining (acridine orange/ethidium bromide) and morphological changes. Conclusion: Different stages of apoptosis revealed the importance of A.paniculata in hepatocellular carcinoma cell lines. Andrographolide and analogues have been subjected to extensive chemico- biological investigations for anticancer drug development. Several andrographolide analogues have shown superior anticancer activities in both in vitro and in vivo models. Further clinical and biomedical studies are required to confirm the pharmacological, pharmaceutical and toxicological properties of andrographolide.