Skip to content
Research Article Open access CC BY 4.0

Evaluating the Effect of Gamma Irradiation and Steam Sterilization on the Survival and Growth of Composted Sawdust Fungi in Ghana

N. K. Kortei, G. T. Odamtten, M. Obodai, V. Appiah, M. Wiafe-Kwagyan

Microbiology Research Journal International · pp. 180–192 · Published 24 Mar 2015

10.9734/BMRJ/2015/16521

Abstract

The growth and survival of some fungi associated with composted and pretreated sawdust particularly for mushroom cultivation were investigated on two growth media; Cooke’s and Oxytetracycline Glucose Yeast Extract (OGYE). Some fungi were isolated during the composting of sawdust over a period of 28 days as well as after pretreatment with gamma irradiation doses of 5, 10, 15, 20, 25 and 32 kGy and moist heat of 100±2ºC for 2.5 hours. Fungal counts ranged 4.72- 5.77 log10 CFU/g and 3.4- 4.1 log10 CFU/g respectively for both media. Both pretreatment methods effectively reduced (p<0.05) fungal counts by an average of 1.48 (irradiation) and 2.22 (steam) log-cycle reductions on OGYE while there was an average of 3.13 (irradiation) and 1.10 (steam) log-cycle reduction on Cooke’s. Corresponding radiation sensitivities (D10 values) of 5.94±2.06 kGy and 5.64±1.12 kGy were recorded for fungi on both media respectively. Five species belonging to three genera were isolated on OGYE and among the fungi were Aspergillus niger, Aspergillus fumigatus, Aspergillus ustus, Mucor racemosus and Rhizopus stolonifer. Ten species belonging to four genera were also isolated on Cooke’s medium included Aspergillus niger, Aspergillus fumigatus, Aspergillus flavus, Aspergillus ustus, Aspergillus terreus, Aspergillus parasiticus, Aspergillus alutaceus, Rhizopus stolonifer, Fusarium oxysporum and Mucor racemosus. There was an observed reduction (p<0.05) in species diversity after pretreatment. The presence of these microorganisms on sawdust also has serious repercussions on animal and human health.

Composting gamma radiation sawdust steam D10 value fungi mushroom

References (34)

  1. 1 Impact of Culture-Independent Studies on the Emerging Phylogenetic View of Bacterial Diversity [DOI]
  2. 2 Integrated Solid Waste Management: Engineering Principles and Management Issues
  3. 3 Biodegradation of lignin in a compost environment: a review [DOI]
  4. 4 Stable-isotope probing as a tool in microbial ecology [DOI]
  5. 5 Microbiological aspects of biowaste during composting in a monitored compost bin [DOI]
  6. 6 Succession of Microbial Communities during Hot Composting as Detected by PCR–Single-Strand-Conformation Polymorphism-Based Genetic Profiles of Small-Subunit rRNA Genes [DOI]
  7. 7 Comparative study on the growth and yield of Pleurotus ostreatus mushroom on different lignocellulosic by-products [DOI]
  8. 8 Microbial activities during composting of spent pig-manure sawdust litter at different moisture contents [DOI]
  9. 9 Isolation and identification of fungal communities in compost and vermicompost [DOI]
  10. 10 Overview of Personal Occupational Exposure Levels to Inhalable Dust, Endotoxin, β(1→3)-Glucan and Fungal Extracellular Polysaccharides in the Waste Management Chain [DOI]
  11. 11 UV spectroscopy: a tool for monitoring humification and for proposing an index of the maturity of compost [DOI]
  12. 12 Potential applications of ionizing radiation in postharvest handling of fresh fruits and vegetables
  13. 13 Themes for mushroom exploitation in the 21st century: Sustainability, waste management, and conservation. [DOI]
  14. 14 Characteristics of rice straw and sewage sludge as composting materials in Valencia (Spain) [DOI]
  15. 15 The use of microorganisms in environmental remediation.
  16. 16 Influence of composting techniques on microbial succession, temperature and pH in a composting municipal solid waste
  17. 17 Microbiological Quality of Chicken Sold in Accra and Determination of D10-Value of E. coli [DOI]
  18. 18 Radiosensitization of Aspergillus niger and Penicillium chrysogenum using basil essential oil and ionizing radiation for food decontamination [DOI]
  19. 19 Chemical, Physical and Microbiological Changes during Composting of the Water Hyacinth [DOI]
  20. 20 Succession of microfungal assemblages in decomposing peatland plants [DOI]
  21. 21 Applied aspects of composting and bioconversion of lignocellulosic materials: An overview [DOI]
  22. 22 Optimisation of solid substrate fermentation of wheat straw into animal feed by Pleurotus ostreatus: a pilot effort [DOI]
  23. 23 Preliminary investigations into the bioconversion of gamma irradiated agricultural waste by Pleurotus spp. [DOI]
  24. 24 Microbiological quality assessment of gamma irradiated fresh and dried mushrooms ( Pleurotus ostreatus ) and determination of D10 values of Bacillus cereus in storage packs.
  25. 25 Physical, chemical and fungal phenology associated with the composting of 'wawa' sawdust (Triplochiton scleroxylon) used in the cultivation of oyster mushrooms in Ghana
  26. 26 Mycoflora Associated with Decomposition of Rice Stubble Mixed with Soil [DOI]
  27. 27 The use of antibiotics in media for the isolation of fungi from polluted water.
  28. 28 Improvement of shelf-life and postharvest quality of white button mushroom by 60Co gamma-ray irradiation
  29. 29 COMPARATIVE EFFECT OF GAMMA IRRADIATED AND STEAM STERILIZED COMPOSTED 'WAWA' (TRIPLOCHITON SCLEROXYLON ) SAWDUST ON THE GROWTH AND YIELD OF PLEUROTUS OSTREATUS (JACQ. EX. FR) KUMMER
  30. 30 Mycoflora associated with paddy straw substrate during different stages and cropping months of Oyster mushroom (Pleurotus florida)
  31. 31 Cellulolytic activity of pure and mixed cultures of fungi [DOI]
  32. 32 Cultivation of mushroom (Pleurotus ostreatus) and the microorganisms associated with the substrate used
  33. 33 Effect of oil (crude petroleum) on the survival and growth of soil fungi
  34. 34 Microbial diversity in the municipal composting process and development of detection methods

Cited by 12

Identification of Fungi Associated with Strawberry Fruits Taken from Sulaimani Markets and their In Vitro Management Techniques

A. Mirza, J. H. Ismael, Sidiq A. Sidiq Kasnazany · Tikrit Journal for Agricultural Sciences · 2021

Combined effects of gamma-irradiation, dose rate and mycobiota activity on cultural heritage – Study on model paper

Katarina Marušić, M. Klarić, Lucija Sinčić · Radiation Physics and Chemistry · 2020

INFLUENCE OF GAMMA-RADIATION ON THIRD AND FOURTH GENERATION CEPHALOSPORIN ANTIBIOTICS

M. A. M. Abo-State, M. Yassin, Marwa Mohammed El-Shora · 2019

Identification of Fungi Associated with Strawberry Fruits Taken from Sulaimani Markets and their In Vitro Management Techniques

Avin Noori Mirza, Jalal H. S. Ismael, Sidiq A. Sidiq kasnazany · Tikrit Journal for Agricultural Sciences · 2021

Showing 10 of 12 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

75

Page views

0

PDF downloads

45

Outbound clicks

12

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.