Efficacy of Disinfectants on Candida Biofilms at Different Concentrations and Contact Times
Yasemin Oz, Ilknur Dag, Nuri Kiraz
Microbiology Research Journal International · pp. 40–52 · Published 2 May 2012
10.9734/BMRJ/2012/1281Abstract
Aims: Candida species cause a wide spectrum of diseases, including hospital-acquired and device-associated infections. The biofilm formation is a major virulence factor in Candida pathogenesis and the cells in biofilm show enhanced resistance to disinfectants. Our aim was to evaluate the efficiency of the commonly used hospital disinfectants (glutaraldehyde (GLU), hydrogen peroxide (HP), peracetic acid (PA), ortho-phtalaldehyde (OPA) and sodium hypochlorite (SH) on biofilms of clinical Candida (C. albicans, C. glabrata, C. parapsilosis, C. krusei and C. tropicalis) isolates. Study Design: An experimental study. Place and Duration of Study: Department of Microbiology, Faculty of Medicine and Electron Microscope Laboratory, Eskisehir Osmangazi University, between January 2011 and May 2011. Methodology: These disinfectants were selected due to their common application in hospital environment. Their concentrations were adjusted to manufacturer’s recommendations for instrument disinfection: 5% HP, 0.2% PA, 5.25% SH (5000 ppm of chlorine), 2% GLU and 0.55% OPA. They were also prepared at the 1:2 and 1:4 times of recommended concentration to evaluate the activity of lower concentrations. The biofilms were grown in microplates and treated with disinfectants at contact times 1, 5 and 10 minutes (20 min for GLU), then stained with the biomass indicator (2, 3-Bis [2-methoxy-4-nitro-5-(sulfenylamino) carbonyl-2H-tetrazolium-hydroxide]). Results: The disinfectants reduced the biofilm for all concentrations studied, however none of them completely removed the biofilm. When they were used at low concentration, longer contact times were more effective. However, when the disinfectants were used in recommended concentration, results showed many variations depending on the disinfectant type, contact times and species. Conclusion: Our results also emphasize the importance of regular disinfection, before the starting of biofilm formation.
References (25)
- 1 Biofilms: Survival Mechanisms of Clinically Relevant Microorganisms [DOI]
- 2 Guideline for disinfection and sterilization in healthcare facilities, 2008
- 3 Candida biofilms and their role in infection [DOI]
- 4 Healthcare Infection Control Practices Advisory Committee
- 5 Inhibition of Candida albicans Biofilm Formation by Farnesol, a Quorum-Sensing Molecule [DOI]
- 6 Biofilm formation by Candida species on the surface of catheter materials in vitro [DOI]
- 7 Similarities and differences in the responses of microorganisms to biocides [DOI]
- 8 Biofilm Production by Candida Species and Inadequate Antifungal Therapy as Predictors of Mortality for Patients with Candidemia [DOI]
- 9 Biofilm Production by Isolates of Candida Species Recovered from Nonneutropenic Patients: Comparison of Bloodstream Isolates with Isolates from Other Sources [DOI]
- 10 Efficacy of common hospital biocides with biofilms of multi-drug resistant clinical isolates [DOI]
- 11 Fusarium and Candida albicans Biofilms on Soft Contact Lenses: Model Development, Influence of Lens Type, and Susceptibility to Lens Care Solutions [DOI]
- 12 Resistance to Quaternary Ammonium Compounds in Food-Related Bacteria [DOI]
- 13 Efficacy of antiseptics and disinfectants on clinical and environmental yeast isolates in planktonic and biofilm conditions [DOI]
- 14 Microscopical analysis of Candida albicans biofilms on heat-polymerised acrylic resin after chlorhexidine gluconate and sodium hypochlorite treatments [DOI]
- 15 Reduced Biocide Susceptibility in Candida albicans Biofilms [DOI]
- 16 Biofilm Formation and Biocide Susceptibility Testing of Mycobacterium fortuitum and Mycobacterium marinum [DOI]
- 17 [44] Pseudomonas aeruginosa biofilm sensitivity to biocides: Use of hydrogen peroxide as model antimicrobial agent for examining resistance mechanisms [DOI]
- 18 XTT assay for evaluating the effect of alcohols, hydrogen peroxide and benzalkonium chloride on biofilm formation of Staphylococcus epidermidis [DOI]
- 19 Biofilms on environmental surfaces: Evaluation of the disinfection efficacy of a novel steam vapor system [DOI]
- 20 A new microtitre plate screening method for evaluating the viability of aerobic respiring bacteria in high surface biofilms [DOI]
- 21 Resistance of Listeria monocytogenes biofilms to disinfectants 326 [DOI]
- 22 Evaluation of different detection methods of biofilm formation in clinical Candida isolates. [DOI]
- 23 Efficacy of disinfectants against biofilm cells of Pseudomonas aeruginosa.
- 24 The effects of disinfectant foam on microbial biofilms [DOI]
- 25 Investigation of biofilm production by Candida species isolated from various clinical samples [DOI]
Cited by 10
S. Pokhrel, Nawarat Boonmee, Orawan Tulyaprawat · Journal of Fungi · 2022
U. Nemchenko, E. Kungurtseva, E. Grigorova · Russian Clinical Laboratory Diagnostics · 2020
L. Pham, Sujiraphong Pharkjaksu, P. Chongtrakool · Frontiers in Microbiology · 2019
A. Shittu, M. Ja’afaru · 2018
S. Aka, S. H. Haji · Brazilian Journal of Microbiology · 2015
A. Zarei Mahmoudabadi, Majid Zarrin, Neda Kiasat · Jundishapur Journal of Microbiology · 2014
Showing 6 of 10 known citations — external sources report more than can currently be individually listed.
Related research
- Application of a Novel Tongue Resin Replication System for in vitro Biofilm Studies — shares topic coverage
- Recurrent Glaucoma Drainage Device Erosion Associated with Occult Infection with Biofilm-producing Organisms — shares topic coverage
- Phenotypic and Genotypic Detection of Biofilm Formation in Staphylococcus epidermidis Isolates from Retrieved Orthopaedic Implants and Prostheses — shares topic coverage
- Characterization of Vaginal Lactobacillus Strains and Their Potential Antagonistic Effects on Candida albicans — shares topic coverage
- Antifungal Resistance Patterns, Virulence Attributes and Spectrum of Oral Candida Species in Patients with Periodontal Disease — shares topic coverage
Article metrics
Real usage data collected on this platform.
59
Page views
0
PDF downloads
41
Outbound clicks
10
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.