Skip to content
Research Article Open access CC BY 4.0

Efficacy of Ascorbic Acid on Frontal Cortex Damage Induced by Alcoholic Extract of Datura stramonium Leaf in Adult Male Wistar Rats

Atiba Kehinde Peter, Onyemeh Linda Oluchi, Sunday Kolawole Akomolede, Paul Obaloluwa Ojetayo, Patricia Okwuchi Nneji, Aniekwensi Obinna Patrick, Gemadi Kwasi Shine, Adedoyin Tinuade Buari, Olajide Sarah Olulana, Manawa Oghenevware

Asian Journal of Research in Medical and Pharmaceutical Sciences · pp. 34–53 · Published 14 Jun 2024

10.9734/ajrimps/2024/v13i3260

Abstract

Introduction: Datura stramonium (DS) is a medicinal plant widely distributed across the globe, including in Nigeria and West Africa. Pharmacological, physiological, and histological studies have demonstrated the neurotoxicity of the plant in animals and humans. Ascorbic acid is a potent reducing agent and scavenger of  free radicals in biological systems. Aim: This study was undertaken to advance our knowledge on Datura stramonium leaf  toxicity and investigate ascorbic acid efficacy on the frontal cortex damage induced by the alcoholic extract of Datura stramonium leaf  in adult male Wistar rats. Settings and Design: Thirty (30) adults male Wistar rats weighing about 120±20g were divided into six groups (A-F) of five animals each for oral administration over 14 days. Materials and Methods: • Group A (Control): Received only rat feeds and water. • Group B: Received 200 mg/kg alcoholic extract of Datura stramonium. • Group C: Received 400 mg/kg alcoholic extract of Datura stramonium. • Group D: Received 200 mg/kg body weight alcoholic extract of Datura stramonium and 200 mg/kg body weight of Vitamin C. • Group E: Received 400 mg/kg body weight alcoholic extract of Datura stramonium and 200 mg/kg body weight of Vitamin C. • Group F: Received 200 mg/kg body weight of Vitamin C. The experimental animals were euthanized, and sections of the frontal cortex of the brain were harvested for histological procedures, organ weight (brain) and body weight of experimental animals were obtained. The data was subjected to a one-way analysis of variance (ANOVA). Results: Histological observations indicated that the administration of the alcoholic extract of DS leaf in Group C showed degeneration of neurons in the frontal cortex. Groups that received ascorbic acid along with DS leaf in smaller doses showed no significant changes and had normal neuronal cells and stroma. Changes observed in body weight were not statistically significant at p-value <0.05. Conclusion: This study suggests that ascorbic acid effectively reduces the neurotoxicity potential of Datura stramonium on the frontal cortex. The DS leaf extract may have neurodegenerative effects at high doses, and precautions should be taken when consuming DS, as it may adversely affect and damage neurons in the frontal cortex.

Datura stramonium vitamin C neurodegeneration frontal cortex neuron

References (39)

  1. 1 Oxidative Stress in Neurodegenerative Diseases: From a Mitochondrial Point of View [DOI]
  2. 2 Mitochondrial Oxidative Stress—A Causative Factor and Therapeutic Target in Many Diseases [DOI]
  3. 3 Histological Stains: A Literature Review and Case Study [DOI]
  4. 4 Back to Nature: Medicinal Plants as Promising Sources for Antibacterial Drugs in the Post-Antibiotic Era [DOI]
  5. 5 Oxidative Stress, Inflammation, and Disease [DOI]
  6. 6 Vitamin C: An Antioxidant Agent [DOI]
  7. 7 Green synthesis of silver nanoparticles using Datura stramonium leaf extract and assessment of their antibacterial activity [DOI]
  8. 8 Histology and Cell Biology—An Introduction to Pathology [DOI]
  9. 9 Antimicrobial Importance of Medicinal Plants in Nigeria [DOI]
  10. 10 Phytochemistry, Pharmacology, and Toxicology of Datura Species—A Review [DOI]
  11. 11 Trying to Solve the Puzzle of the Interaction of Ascorbic Acid and Iron: Redox, Chelation and Therapeutic Implications [DOI]
  12. 12 Medical importance of Datura fastuosa (syn: Datura metel) and Datura stramonium - A review [DOI]
  13. 13 Basic and Advanced Laboratory Techniques in Histopathology and Cytology [DOI]
  14. 14 Antioxidants [DOI]
  15. 15 Phytochemical Properties and Pharmacological Role of Plants: Secondary Metabolites [DOI]
  16. 16 Basic Histological Techniques for Planarians [DOI]
  17. 17 An Overview of the Characteristics and Function of Vitamin C in Various Tissues: Relying on its Antioxidant Function [DOI]
  18. 18 Potential antidiabetic effect of ethanolic and aqueous-ethanolic extracts of Ricinus communis leaves on streptozotocin-induced diabetes in rats [DOI]
  19. 19 Beyond a rod through the skull: A systematic review of lesion studies of the human ventromedial frontal lobe [DOI]
  20. 20 Datura stramonium essential oil composition and it’s immunostimulatory potential against colon cancer cells [DOI]
  21. 21 Fixation and different types of fixatives: Their role and functions: A review [DOI]
  22. 22 Preparation, construction and high-throughput automated analysis of human brain tissue microarrays for neurodegenerative disease drug development [DOI]
  23. 23 Evaluation of in vitro Antioxidant Activities of Ethanol Extracts of Datura innoxia Mill. Leaves and Seeds Harvested in Mali [DOI]
  24. 24 Pharmacognostic Review on Datura [DOI]
  25. 25 Datura metel stramonium exacerbates behavioral deficits, medial prefrontal cortex, and hippocampal neurotoxicity in mice via redox imbalance [DOI]
  26. 26 Pediatric Sedation Outside of the Operating Room [DOI]
  27. 27 The Medicinal Significance of Datura stramonium: A Review [DOI]
  28. 28 Histological Patterns of Neurodegeneration of Frontal Cortex Neurons in Datura stramonium Treated Wistar Rats [DOI]
  29. 29 An Overview on Datura stramonium L. (Jimson weed): A Notable Psychoactive Drug Plant [DOI]
  30. 30 Neurotoxic Potential of Alkaloids from Thorn Apple (Datura stramonium L.): A Commonly Used Indian Folk Medicinal Herb [DOI]
  31. 31 Phytoconstituents of Datura metel extract improved motor coordination in haloperidol-induced cataleptic mice: Dual-target molecular docking and behavioural studies [DOI]
  32. 32 Datura stramonium L. (Solanaceae) [DOI]
  33. 33 STUDIES ON THE PHYTOCHEMICAL SCREENING AND DELETERIOUS EFFECTS OF AQUEOUS EXTRACT OF DATURA STRAMONIUM ON ALBINO RATS
  34. 34 Evaluation of the Neurobehavioural Toxicity Potential of Aqueous Ethanol Extracts of Leaf/Seed of Datura metel, Mucuna pruriens, and Tapinanthus globiferus Growing on Azadirachta indica Host Tree in Mice [DOI]
  35. 35 Wound healing activity of the hydro-alcoholic extract of Datura stramonium leaves in wistar albino rats. [DOI]
  36. 36 Pro-oxidant effect of vitamin c with chronic monosodium glutamate organ toxicity in albino rats [DOI]
  37. 37 An overview of tropane alkaloids from Datura stramonium L.
  38. 38 Male Reproductive System [DOI]
  39. 39 10.13040/ijpsr.0975-8232.11(7).3123-37 [DOI]

Cited by 1

Identification of Datura metel phytochemicals as potential EGFR inhibitors in lung cancer

Pankaja Pandey, Priyanka Joshi, Shilpi Rawat · Scientific Reports · 2025

Related research

Article metrics

Real usage data collected on this platform.

12

Page views

0

PDF downloads

0

Outbound clicks

1

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.