Skip to content
Research Article Open access CC BY 4.0

Effects of the Combination of Aqueous Extracts of Vernonia amygdalina and Tamarindus indica on Digestive Enzymes and Hematological Profile of Streptozotocin-Induced Diabetic Rats

Tekou Florian Amel, Kemtsop Michel Pegui, Kuate Dieudonné, Dzoyem Jean Paul

Asian Journal of Research in Biochemistry · pp. 1–8 · Published 1 Jan 2025

10.9734/ajrb/2025/v15i1345

Abstract

Aims: Many strategies have been put in place to stop diabetes and its related complications: the use of plants has proved to be less costly and with few side-effects. Among these plants Vernonia amygdalina and Tamarindus indica are used because of their richness in bioactive compounds, notably phenols and dietary fiber. The aim of this work was first to evaluate the effect of combining aqueous extracts of Vernonia amygdalina leaves and Tamarindus indica pulp on two energy metabolism enzymes (alphaamylase and pancreatic lipase), and secondly to assess the effect of combining these extracts on the hematological profile of streptozotocin-induced diabetic wistar rats. Methodology: Vernonia amygdalina leaves and Tamarindus indica pulp was subject to aqueous extraction, the two extracts obtained were mixed in vitro in the presence of the two enzymes and their inhibitory power evaluated; streptozotocin was used to induce diabetes in Wistar rats which were then treated with our extracts and the combination of these; their blood was collected and hematological analysis carried out. Results: Our findings revealed that at 100 mg/l, the mixture showed a 23.56±4.6% inhibition of alphaamylase. The percentage inhibition of pancreatic lipase varied depending on the extract concentration from 8.81±1.90 at 62.5µg/ml to 31.95±1.90 at 1000µg/ml, and the mixture also improved hematological parameters in diabetic rats. Conclusions: combining these two extracts makes it possible to increase the inhibitory power with respect to these enzymes and improves the hematological profile, this could therefore be beneficial for the non drug management of diabetes.

Type 2 diabetes mellitus Vernonia amydalina Tamrindus indica α-amylase pancreatic lipase

References (9)

  1. 1 Anthropometrical parameters and markers of obesity in rats [DOI]
  2. 2 Methods for lipase detection and assay: a critical review [DOI]
  3. 3 Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents of Canarium tramdenum Bark [DOI]
  4. 4 Poorly controlled type 2 diabetes is accompanied by significant morphological and ultrastructural changes in both erythrocytes and in thrombin-generated fibrin: implications for diagnostics [DOI]
  5. 5 The effect of dietary fiber on human pancreatic enzyme activity in vitro [DOI]
  6. 6 Aqueous extract of Tamarindus indica fruit pulp exhibits antihyperglycaemic activity. [DOI]
  7. 7 Oxidative stress in diabetic patients with retinopathy [DOI]
  8. 8 EVALUATION OF THE ANTIOXIDANT AND Α-AMYLASE INHIBITORY ACTIVITIES OF Mitragyna inermis (WILLD) O. KUNTZE AND Tamarindus indica LINN. [DOI]
  9. 9 Pancreas Protective Effect of Ethanolic Extract Vernonia Amygdalina Delile on Rats Induced Doxorubicin [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

36

Page views

0

PDF downloads

14

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.