Skip to content
Research Article Open access CC BY 4.0

Effects of Seed Priming with Proline and Glycine Betaine on Germination, Seedling Growth, and Photosynthetic Pigments of Rice (Oryza sativa) under Chilling Stress

Shaila Shermin Tania, Tania, Shaila Shermin, Mohammad Saidur Rhaman, Hoque, Md Anamul, Md. Moklasur Rahaman, Md Anamul Hoque, Rahaman, Md. Moklasur, Rhaman, Mohammad Saidur

Journal of Agriculture and Ecology Research International · pp. 15–23 · Published 1 Jul 2022

10.9734/jaeri/2022/v23i530235

Abstract

Aims: Rice grown in late Rabi season frequently results poor germination and establishment because of exposure to extreme low temperature or chilling stress. Seed priming is an effective seed treating technique for uniform and good germination in various crops under different abiotic stresses. The present study was undertaken to assess the role of different seed priming agents in seed germination and seedling growth of rice (BRRI Dhan 29) under chilling stress (CS). Place and Duration of Study: a pot experiment was carried out at the Department of Seed Science and Technology, Bangladesh Agricultural University during Late November – Mid December 2021. Methodology: The rice seeds (BRRI Dhan 29) were soaked in different priming agents (hydro-priming, 20 mM proline and glycine betaine) for 30 min. The primed seeds were exposed to cold stress (under 5°C) in the refrigerator. The untreated seeds were used as control. Results: The outcomes shown that proline (Pro) and glycine betaine (GB) priming had a favorable effect on germination and survival rate, shoot and root length, shoot and root weight, and photosynthetic pigments of leaves under CS. Among the treatments, Pro and GB at 20 mM and warm water performed better; while priming with tap water and Pro and GB at a concentration of demonstrated insignificant performance over control but considerable over stress in the majority of the parameters. The findings showed that seed priming generally has a favorable effect on germination and survival, shoot and root length and weight, and leaf chlorophyll content. Conclusion: Proline, betaine, or warm water priming (at 45°C for 5 min.) can be thought of as a lasting priming agent. This means that priming may be an efficient strategy to boost seed germination and better seedling growth of rice under chilling stress.

Germination survival chlorophyll content cold stress

References (30)

  1. 1 [34] Chlorophylls and carotenoids: Pigments of photosynthetic biomembranes [DOI]
  2. 2 Seed priming for abiotic stress tolerance: an overview [DOI]
  3. 3 How Does Proline Treatment Promote Salt Stress Tolerance During Crop Plant Development? [DOI]
  4. 4 ABA Regulates Apoplastic Sugar Transport and is a Potential Signal for Cold-Induced Pollen Sterility in Rice [DOI]
  5. 5 Seed Priming with Phytohormones: An Effective Approach for the Mitigation of Abiotic Stress [DOI]
  6. 6 Physiological and Biochemical Mechanisms of Seed Priming-Induced Chilling Tolerance in Rice Cultivars [DOI]
  7. 7 Rice and cold stress: methods for its evaluation and summary of cold tolerance-related quantitative trait loci [DOI]
  8. 8 Priming and Pretreatment of Seeds and Seedlings [DOI]
  9. 9 Seed Priming with Melatonin Improves the Seed Germination of Waxy Maize under Chilling Stress via Promoting the Antioxidant System and Starch Metabolism [DOI]
  10. 10 Induction of chilling tolerance in wheat during germination by pre-soaking seed with nitric oxide and gibberellin [DOI]
  11. 11 Seed Priming Methods: Application in Field Crops and Future Perspectives [DOI]
  12. 12 5-aminolevulinic acid-mediated plant adaptive responses to abiotic stress [DOI]
  13. 13 Rice seed priming with sodium selenate: Effects on germination, seedling growth, and biochemical attributes [DOI]
  14. 14 The effect of hydro and proline seed priming on growth, proline and sugar content, and antioxidant activity of maize under cadmium stress [DOI]
  15. 15 Physiological and biochemical responses to the exogenous application of proline of tomato plants irrigated with saline water [DOI]
  16. 16 Alleviation of Salt-Inhibited Germination and Seedling Growth of Kidney Bean by Seed Priming and Exogenous Application of Salicylic Acid (SA) and Hydrogen Peroxide (H2O2) [DOI]
  17. 17 Germination and radicle growth in unprimed and primed seeds of sweet sorghum as affected by reduced water potential in NaCl at different temperatures [DOI]
  18. 18 Seed priming with proline improved photosystem II efficiency and growth of wheat (Triticum aestivum L.) [DOI]
  19. 19 Climate Change and Its Adverse Impacts on Plant Growth in South Asia: Current Status and Upcoming Challenges [DOI]
  20. 20 Cold Injury and Flash Flood Damage in Boro Rice Cultivation in Bangladesh: A Review [DOI]
  21. 21 Hydro-priming and halo-priming improve seed germination, yield and yield contributing characters of Okra (Abelmoschus esculentus L.) [DOI]
  22. 22 Cadmium Toxicity Alleviates by Seed Priming with Proline or Glycine Betaine in Cowpea ( Vigna unguiculata (L.) Walp.) [DOI]
  23. 23 Seed priming with Salicylic Acid (SA) and Hydrogen Peroxide (H2O2) Improve Germination and Seedling Growth of Wheat (Triticum aestivum) under Salt Stress [DOI]
  24. 24 SEED PRIMING AND EXOGENOUS APPLICATION OF SALICYLIC ACID ENHANCE GROWTH AND PRODUCTIVITY OF OKRA (Abelmoschus esculentus L.) BY REGULATING PHOTOSYNTHETIC ATTRIBUTES [DOI]
  25. 25 Seed treatment with glycine betaine enhances tolerance of cotton to chilling stress [DOI]
  26. 26 Effects of hydropriming on seed germination, seedling growth and yield of bitter gourd [DOI]
  27. 27 Optimum sowing date and cultivar duration of dry-seeded boro on the High Ganges River Floodplain of Bangladesh [DOI]
  28. 28 Seed Priming Toward Enhanced Chilling Tolerance in Field Crops: An Overview [DOI]
  29. 29 Environmental science and pollution research [DOI]
  30. 30 Examining the physiological and genetic response of maize to low temperature conditions

Cited by 13

Mitigation of Salinity-Induced Growth Inhibition of Maize by Seed Priming and Exogenous Application of Salicylic Acid

Md. Ahsan Ullah, Ashaduzzaman Sagar, Md. Ashik Mia, Shaila Shermin Tania, Jannat-E-Tajkia, Muhammad Humayun Kabir, A. K. M. Zakir Hossain, Farjana Rauf, Mohammad Saidur Rhaman · Journal of Agriculture and Ecology Research International · 2023

Seed priming with osmolytes improves germination characteristics and plant growth attributes under abiotic stresses

Shifa Shaffique, Raheem Shahzad, Javier A. Trujillo-Rugamas · Roles of Osmolytes in Changing Environment · 2025

Proline and glycine betaine: A dynamic duo for enhancing salt stress resilience in maize by regulating growth, Stomatal size, and Oxidative stress responses

Mohammad Saidur Rhaman, Farjana Rauf, Shaila Shermin Tania · Plant Stress · 2024

The effect of different proline and glycine concentrations on the growth activity of nodule bacteria Bradyrhizobium japonicum in vitro and the germination of soybean seeds

Institute of Plant Physiology and Genetics, National Academy of Sciences of Ukraine 31/17 Vasylkivska St., Kyiv, 03022, Ukraine, S.Ya. Kots, A.V. Khrapova · Fiziologia rastenij i genetika · 2024

IMPROVING COLD STRESS TOLERANCE IN RICE SEEDLINGS

Md. TAHJIB-UL-ARIF · Journal of Environmental Science for Sustainable Society · 2023

Showing 5 of 13 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

59

Page views

0

PDF downloads

20

Outbound clicks

13

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.