Effects of Mesenchymal Stem Cells and Their Derived Microvesicles on Pulmonary Toxicity Induced by Petrol Exhaust Nanoparticle; Histological and Immuno-Histochemical Study
Sherifa Abd El Salam, Eman Mohamed Faruk, Hanan Fouad, Naglaa Yehia Nafie
Annual Research & Review in Biology · pp. 1–14 · Published 4 Jun 2019
10.9734/arrb/2019/v31i630070Abstract
Background: Diesel vehicles exhaust contains toxic nanoparticles that drastically affect lung tissue due to their direct cytotoxic effects, induction of oxidative stress, inflammatory signaling pathways and DNA damage. Mesenchymal stem cells (MSCs) exhibit anti-inflammatory effects and efficient regenerative capacity in chronic lung diseases. Objectives: Evaluation of the effects of MSCs and MSCs-derived micro vesicles (MSCs-MVs) on pulmonary toxicity induced by diesel exhaust nanoparticles (DENPs). Materials and Methods: Sixty male rats were equally divided into: Group I (Control rats), Group II (DENPs group) received repeated doses of DENPs (180μg/rat) intratracheally every other day for 6 days, Group III (MSCs group) received MSCs intravenously (3×106 cells) after the last dose of DENPs and Group IV (MSCs-MVs group) received MSCs-MVs (0.5 mg/mL) intravenously after the last dose of DENPs. Lung tissue were subjected to histological and immunohistochemical assessment. Inflammatory cytokines and bronchoalveolar lavage fluid (BALF) contents of inflammatory cells, albumin, LDH and total proteins were evaluated. Results: Histological picture of lung tissue in DENPs group showed numerous collapsed alveoli, thick interalveolar septa and marked cellular infiltration. Elastic fibers were markedly decreased by DENPs. Increased optical density of NF-κB/p65 immunoreactivity. Bronchoalveolar lavage fluid showed significant elevation of inflammatory cytokines (TNF-a, IL-6), polymorphonuclear leukocytes (PMN), neutrophils, macrophages, LDH, total proteins and albumin. Treatment with either MSCs or MSCs-MVs led to a significant amelioration of all of the aforementioned studied parameters. Conclusion: MSCs-MVs and MSCs showed significant therapeutic effects against DENPs damaging effects on the lung tissues via their regenerative capacity and anti-inflammatory effects.
References (34)
- 1 Biological properties of extracellular vesicles and their physiological functions [DOI]
- 2 Concise Review: MSC-Derived Exosomes for Cell-Free Therapy [DOI]
- 3 Extracellular vesicles derived from human bone marrow mesenchymal stem cells promote angiogenesis in a rat myocardial infarction model [DOI]
- 4 Mesenchymal Stromal Cell Exosomes Ameliorate Experimental Bronchopulmonary Dysplasia and Restore Lung Function through Macrophage Immunomodulation [DOI]
- 5 Mesenchymal Stem Cell Transplantation Reverses Multiorgan Dysfunction in Systemic Lupus Erythematosus Mice and Humans [DOI]
- 6 Umbilical cord mesenchymal stem cells derived extracellular vesicles can safely ameliorate the progression of chronic kidney diseases [DOI]
- 7 Histological Stains: A Literature Review and Case Study [DOI]
- 8 The hematoxylins and eosin [DOI]
- 9 Diesel exhaust: current knowledge of adverse effects and underlying cellular mechanisms [DOI]
- 10 Extracellular vesicle transfer of cancer pathogenic components [DOI]
- 11 Endothelial-to-mesenchymal transition: Cytokine-mediated pathways that determine endothelial fibrosis under inflammatory conditions [DOI]
- 12 DNA damage response in adult stem cells: pathways and consequences [DOI]
- 13 Induction of pulmonary fibrosis by cerium oxide nanoparticles [DOI]
- 14 Isolation, differentiation, and characterization of mesenchymal stem cells from human bone marrow.
- 15 Mesenchymal Stem Cell Therapy for Inflammatory Skin Diseases: Clinical Potential and Mode of Action [DOI]
- 16 Regulated and Unregulated Emissions from Highway Heavy-Duty Diesel Engines Complying with U.S. Environmental Protection Agency 2007 Emissions Standards [DOI]
- 17 Mesenchymal stem cells and their therapeutic applications in inflammatory bowel disease [DOI]
- 18 Cerium Oxide Nanoparticles in Lung Acutely Induce Oxidative Stress, Inflammation, and DNA Damage in Various Organs of Mice [DOI]
- 19 DNA double‐strand break response in stem cells: Mechanisms to maintain genomic integrity [DOI]
- 20 Inhaled Diesel Emissions Generated with Cerium Oxide Nanoparticle Fuel Additive Induce Adverse Pulmonary and Systemic Effects [DOI]
- 21 Effects of ultrafine petrol exhaust particles on cytotoxicity, oxidative stress generation, DNA damage and inflammation in human A549 lung cells and murine RAW 264.7 macrophages [DOI]
- 22 Anti-inflammatory and antioxidant effects of mesenchymal and hematopoietic stem cells in a rheumatoid arthritis rat model [DOI]
- 23 Protective Role of Catechin and Quercetin in Sodium Benzoate-Induced Lipid Peroxidation and the Antioxidant System in Human ErythrocytesIn Vitro [DOI]
- 24 Bronchoalveolar Lavage (BAL) for Research; Obtaining Adequate Sample Yield [DOI]
- 25 Biopersistence of NiO and TiO2 Nanoparticles Following Intratracheal Instillation and Inhalation [DOI]
- 26 Genotoxic potential of diesel exhaust particles from the combustion of first- and second-generation biodiesel fuels—the FuelHealth project [DOI]
- 27 Isolation and Flow Cytometry Characterization of Extracellular‐Vesicle Subpopulations Derived from Human Mesenchymal Stromal Cells [DOI]
- 28 Protein composition of bronchoalveolar lavage fluid and airway surface liquid from newborn pigs [DOI]
- 29 Human induced pluripotent stem cell‑derived mesenchymal stem cells alleviate atherosclerosis by modulating inflammatory responses [DOI]
- 30 Antitumoral potential of microvesicles extracted from human adipose-derived mesenchymal stem cells on human breast cancer cells [DOI]
- 31 Balancing self-renewal against genome preservation in stem cells: How do they manage to have the cake and eat it too? [DOI]
- 32 PROTECTIVE ROLE OF FENUGREEK LEAF EXTRACT AND QUERCETIN AGAINST PETROL EXHAUST NANOPARTICLE INDUCED LIPID PEROXIDATION AND OXIDATIVE STRESS IN RAT ERYTHROCYTES IN VITRO
- 33 Does Exosomes Derived Bone Marrow Mesenchymal Stem Cells Restore Ovarian Function by Promoting Stem Cell Survival on Experimentally Induced Polycystic Ovary in Adult Female Albino Rats? (Histological and Immunohistochemical Study) [DOI]
- 34 The possible protective role of quercetin on induced cardiac Oxidative DNA Damage by repeated exposure to diesel exhaust nanoparticles in rats (a histological and immunohistochemical study) [DOI]
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