Skip to content
Research Article Open access CC BY 3.0

Effect of Some Physical Factors on Natural Biosynthesis by Streptomyces spp

Nazar Edward Nasser

Current Journal of Applied Science and Technology · pp. 2762–2771 · Published 15 May 2014

10.9734/BJAST/2014/8614

Abstract

Aims: This study was performed to detect the effects of certain environmental conditions mostly physical ones on the biosynthesis of secondary metabolites produced by Streptomyces spp. Study Design: Interventional study. Place and Duration of the Study: Department of biology, College of science, Al-Mustansiriya University, Baghdad ,Iraq between February 2012 and December 2012. Methodology: eight pure Streptomyces isolates, that produce antibiotics ,were exposed to different ranges of temperature, pH, aeration and radiation to elicit the optimum physical conditions for the biosynthesis of their secondary metabolites by estimating the diameter of inhibition zone of the growth on an isolate of E. coli on the nutrient broth and nutrient agar media after it`s inoculation with these products compared to control ones. Results: The optimum growth temperature, pH, and aeration for the growth of Streptomyces were (28-32)ºC, (6.5-7.5) and 250 RPM respectively. Exposure to radiation for a period of 10 minutes had a lethal effect on Streptomyces but various responses were noticed upon exposure to radiant for less than 5 minutes depending on the species kind. Conclusion: The production of antibiotics as a secondary metabolites by Streptomyces species could be enhanced by optimizing the physical factors affecting their growth like Temperature, pH, aeration and radiation.

Streptomyces temperature pH aeration radiation

References (13)

  1. 1 Antibiotics produced by Streptomyces [DOI]
  2. 2 Genome-minimized Streptomyces host for the heterologous expression of secondary metabolism [DOI]
  3. 3 How antibiotics can make us sick: the less obvious adverse effects of antimicrobial chemotherapy [DOI]
  4. 4 Mycelium Differentiation and Antibiotic Production in Submerged Cultures ofStreptomyces coelicolor [DOI]
  5. 5 Bacterial killing in gastric juice – effect of pH and pepsin on Escherichia coli and Helicobacter pylori [DOI]
  6. 6 Stationary-phase mutation in the bacterial chromosome: Recombination protein and DNA polymerase IV dependence [DOI]
  7. 7 Isolation of soil Streptomyces as source antibiotics active against antibiotic-resistant bacteria
  8. 8 Effect of gamma irradiation on viability and DNA of Staphylococcus epidermidis and Escherichia coli [DOI]
  9. 9 Chapter 4 Analyzing the Regulation of Antibiotic Production in Streptomycetes [DOI]
  10. 10 Sparsomycin Antibiotic Production by Streptomyces Sp. AZ-NIOFD1: Taxonomy, Fermentation, Purification and Biological Activities
  11. 11 Effects of sub-minimum inhibitory concentration antibiotic levels and temperature on growth kinetics and outer membrane protein expression in Mannheimia haemolytica and Haemophilus somnus.
  12. 12 Dynamics of Soil Flora and Fauna in Biological Control of Soil Inhabiting Plant Pathogens [DOI]
  13. 13 Biosynthesis of antibiotic through metabolism of actinomycetes strain MH-9 through shake flask fermentation.

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

57

Page views

0

PDF downloads

16

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.