Effect of Quercetin and Pectinase on Spore Germination and Hyphal Growth of Arbuscular Mycorrhizal Fungi and Biomass Production of Nicotiana tabacum L. and Calopogonium mucunoides desv
E. L. M. Ngonkeu, D. Nwaga, R. Tsetchoua, A. Mogo, A. F. Mongoue, T. A. N. Mballa, L. Manet, O. Boyomo
Annual Research & Review in Biology · pp. 1–11 · Published 18 Mar 2017
10.9734/ARRB/2017/32061Abstract
The aim of this study was to evaluate the ability of mycorrhizal fungi to rapidly colonize roots of host plants and to predict their competitive advantage over native strains. Different concentrations of quercetin and pectinase were used to stimulate hyphal growth and to determine the germination rate of arbuscular mycorrhizal fungi (AMF) in a Petri dish. The best strains were used to inoculate two host plants under green house conditions in the presence of high concentrated polyphenol and enzyme. The essays were carried out in the laboratory of biotechnology center; University of Yaounde I. Five strains of AMF from Cameroonian’s soils were identified and compared with exotic strain. Water agar 0.7% was mixed with three different concentrations of quercetin (0, 2.5 and 5 µM) and pectinase (0, 1.6 and 3.2 units/ml). Nicotiana tabacum and Calopogonium mucunoides were used to determine the root colonisation percent of AMF and shoot biomass after inoculation. Results showed that for most species of AMF, 5% quercetin and 3.2 units/ml of pectinase significantly stimulate in vitro spore germination and hyphal growth. Treatment combining quercetin and pectinase could lead to a synergetic effect on mycorrhizal symbiosis and plant performance. These compounds which can modify the symbiosis physiology may be of great importance in the regulation of plant and soil microbe interaction. They could also contribute in stimulating the growth of these strict biotrophic fungi under in vitro culture, and to enhance an increase in plant biomass.
References (18)
- 1 Spores of mycorrhizal Endogone species extracted from soil by wet sieving and decanting [DOI]
- 2 Induction of antioxidant enzymes is involved in the greater effectiveness of a PGPR versus AM fungi with respect to increasing the tolerance of lettuce to severe salt stress [DOI]
- 3 Intense competition between arbuscular mycorrhizal mutualists in an in vitro root microbiome negatively affects total fungal abundance [DOI]
- 4 Fungal Growth Stimulation by CO 2 and Root Exudates in Vesicular-Arbuscular Mycorrhizal Symbiosis [DOI]
- 5 Flavonoids Released Naturally from Alfalfa Promote Development of Symbiotic Glomus Spores In Vitro [DOI]
- 6 Flavonoids from shoots and roots of Trifolium repens (white clover) grown in presence or absence of the arbuscular mycorrhizal fungus Glomus intraradices [DOI]
- 7 The effect of VA mycorrhizal fungi, phosphorus and drought stress on the growth of Acacia nilotica and Leucaena leucocephala seedlings [DOI]
- 8 Climate, plant response and development of dunes on barrier beaches along the U.S. east coast [DOI]
- 9 The effects of hydroxy fatty acids on the hyphal branching of germinated spores of AM fungi [DOI]
- 10 Inoculation with selenobacteria and arbuscular mycorrhizal fungi to enhance selenium content in lettuce plants and improve tolerance against drought stress [DOI]
- 11 Arbuscular mycorrhizal fungi (AMF) as a biofertilizer - a review.
- 12 Flavonoids released by carrot (Daucus carota) seedlings stimulate hyphal development of vesicular-arbuscular mycorrhizal fungi in the presence of optimal CO2 enrichment [DOI]
- 13 Hydrolytic enzymes and ability of arbuscular mycorrhizal fungi to colonize roots [DOI]
- 14 Glycosidation of apigenin results in a loss of its activity on different growth parameters of arbuscular mycorrhizal fungi from the genus Glomus and Gigaspora [DOI]
- 15 Réponse du fonio blanc ( Digitaria exilis Stapf) à l’inoculation avec des champignons mycorhiziens à arbuscules en conditions semi-contrôlées [DOI]
- 16 Cellular and genetical aspects of interactions between hosts and fungal symbionts in mycorrhizae [DOI]
- 17 Hyphal in vitro growth of the arbuscular mycorrhizal fungus Glomus mosseae is affected by chitinase but not by β-1,3-glucanase [DOI]
- 18 Enhancement of in vitro spore germination of Gigaspora rosea by a cellulase preparation [DOI]
Cited by 6
Vyacheslav S. Mikheev, Irina V. Struchkova, Ludmila M. Churkina · Journal of Fungi · 2023
Tanya Singh, Puneet Singh Chauhan · Flavonoids for Plant Development and Stress Tolerance · 2026
Laura Yesenia Solís-Ramos, Antonio Andrade-Torres, Martin Hassan Polo-Marcial · Agriculture · 2023
Showing 3 of 6 known citations — external sources report more than can currently be individually listed.
Related research
- Seasonal Variation of Mycorrhizal Association (Root Colonization and Spore Density) in Selected Industrial Sites in Kota District of Rajasthan, India — shares topic coverage
- Fungal Communities in Roots, Soil Inhabiting Nematodes and Physico-chemical Parameters of Soils in Three Farms of Commercial Strawberry Production in Moulay Bousselham (Morocco) — shares topic coverage
- Calcofluor white, an Alternative to Propidium Iodide for Plant Tissues Staining in Studies of Root Colonization by Fluorescent-tagged Rhizobia — shares topic coverage
- Identification and Characterization of Arbuscular Mycorrhizal Strains from the Roots and Rhizospheres of Tomato, Brinjal, Chilli and Onion — shares topic coverage
- Field-scale Variation in Infective Potential and Root Colonization of Indigenous Arbuscular Mycorrhizal Fungi Associated with Plantain in Central-West Côte d’Ivoire — shares topic coverage
Article metrics
Real usage data collected on this platform.
58
Page views
0
PDF downloads
29
Outbound clicks
6
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.