Effect of Different Plant Growth Regulators on In-vitro Micropropagation of Banana Cultivar Grand Naine (Musa spp.)
Jyoti Singh, R.S Sengar, Mata Prasad, Ankit Kumar
Journal of Advances in Biology & Biotechnology · pp. 90–98 · Published 8 Mar 2024
10.9734/jabb/2024/v27i3724Abstract
Banana (Musa sapientum) is one of the most important fruit crop cultivated in India. It is widely grown in more than 150 countries, producing on an average of 105 million tons of fruit per annum. Grand naine Banana variety is the most commonly cultivated banana with high yielding plantain. This variety mainly grown in the region of Uttar Pradesh state. Present study mainly focuses on the in-vitro micropropagation method which is described for banana cv. Grand naine. For this study, healthy sword suckers of three months old banana plantlets was used as explants. The explants was sterilized thoroughly and cultured on MS (Murashige and Skoog) media supplemented with the different concentration and combination of auxin and cytokinin like BAP, IAA and NAA which were used for the optimization of best plant growth regulator combination for multiple shoot generation and shoot growth. Among all the tested cytokinin 5 mg/l BAP along with 1mg/l IAA in medium concentration performed well and produced healthy shoots further at higher concentration of all tested cytokinin showed declining effect within the growth parameters. For the rooting purpose, MS media was supplemented with different levels of IBA, IAA and NAA. Results indicated that rooting was highly promoted in the medium supplemented with 0.5 mg/L IAA + 1.5 mg/L IBA.
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Cited by 7
Nino Murvanidze, Karel Doležal, Lenka Plačková · Horticulturae · 2024
Gabriel Faria Parreiras de Andrade, Gabriella Aguiar dos Santos, Vinícius Henrique Moreira · Plant Cell, Tissue and Organ Culture (PCTOC) · 2025
Dianne M. Skogerboe, Kathleen M. Yeater, Maria M. Jenderek · In Vitro Cellular & Developmental Biology - Plant · 2025
Showing 3 of 7 known citations — external sources report more than can currently be individually listed.
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