Skip to content
Research Article Open access CC BY 4.0

Effect of Bacillus pumilus Strains on Heavy Metal Accumulation in Lettuce Grown on Contaminated Soil

Sabeen Alam, Mehboob Alam, Hafsa Naeem, Muhammad Adeel, Adil Riaz, Usama Zeb Awan, Abdul Basit, Burhan Ullah

Asian Journal of Research in Crop Science · pp. 10–20 · Published 10 Apr 2024

10.9734/ajrcs/2024/v9i2262

Abstract

Increasing number of heavy metal on land needs to be addressed through sustainable ways and various species of Bacillus can be used to mitigate heavy metals. The research work entitled “Effect of Bacillus pumilus strains on heavy metal accumulation in lettuce grown on contaminated soil” focuses on functional role of Bacillus pumilus strains inoculated with lettuce seed in mitigating heavy metal present in chromite mining soil. This experiment was conducted at ornamental horticulture nursery, the University of Agriculture Peshawar. In this experiment, factor A was three Bacillus pumilus strains (sequence C-2PMW-8, C-1 SSK-8 and C-1 PWK-7) while soil used for this experiment was collected from Prang Ghar mining site and lettuce seeds were grown in three levels of chromite mining soil (2.27, 4.65 and 7.14 %).The experimental design used during this research was randomized complete block design with two factors and was replicated thrice. The collected data related to effect of chromite mining soil and Bacillus pumilus strains indicated that these both factors have significant influence on growth parameters. For mining soil minimum days to germinate (14 days) was noted in lettuce grown on garden soil inoculated with sequence. Maximum germination percentage noted was (100%) for C-1 SSK-8 grown on garden soil, dry weight of lettuce leaf (5.0 g) for lettuce inoculated with C-1 SSK-8 and C-1 PWK-7 strains, survival percentage (77.9%) for C-1 SSK-8 treated lettuce for sequence C-2 PMW-8. Maximum germination percentage noted was (88%) for garden soil, fresh leaf weight (17.3g), dry leaf weight (6.5g), number of leaf per plant (16), leaf area (49.0 cm2) were noted in garden soil with no chromite mining soil. Results related to heavy metals accumulation showed that minimum chromium was (2.3 mg kg-1) in lettuce and (1.9 mg kg-1) in soil for all three sequences. It can be concluded that chromite mining soil significantly reduced the growth and survival of lettuce, but when lettuce was inoculated with Bacillus.pumilus strains it enhances the growth and survival. Similarly, minimum heavy metal accumulation in plant and soil, regardless of type of B. pumilus used, all three sequences has same mitigating effect on heavy metal in both soil and lettuce. All the three Bacillus pumilus strains ensured reduction in heavy metals content (Cr) in lettuce, below the maximum permissible limits of WHO/FAO 2011.

Bioremediation lettuce heavy metal permissible limits and Bacillus strains Bacillus pumilus atomic density soil modification

References (10)

  1. 1 Heavy Metal Stress, Signaling, and Tolerance Due to Plant-Associated Microbes: An Overview [DOI]
  2. 2 Bacillus species enhance growth parameters of chickpea (Cicer arietinum L.) in chromium stressed soils [DOI]
  3. 3 Evaluating heavy metal accumulation and potential health risks in vegetables irrigated with treated wastewater [DOI]
  4. 4 Enhancing phytoremediation of chromium-stressed soils through plant-growth-promoting bacteria [DOI]
  5. 5 Tolerance and Reduction of Chromium(VI) by Bacillus sp. MNU16 Isolated from Contaminated Coal Mining Soil [DOI]
  6. 6 Bacillus pumilus promotes the growth and nitrogen uptake of tomato plants under nitrogen fertilization [DOI]
  7. 7 Genomic analysis of a ginger pathogen Bacillus pumilus providing the understanding to the pathogenesis and the novel control strategy [DOI]
  8. 8 Accumulation of heavy metals by lettuce (Lactuca sativa L.) irrigated with different levels of wastewater of Quetta city.
  9. 9 Approaches in Advanced Soil Elemental Extractability: Catapulting Future Soil–Plant Nutrition Research [DOI]
  10. 10 The Significance of Bacillus spp. in Disease Suppression and Growth Promotion of Field and Vegetable Crops [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

24

Page views

0

PDF downloads

3

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.