Effect of Angiotensin Receptor Blocker and Probiotics in Caffeine-Induced Anxiety Model

Abstract

Aim:  To evaluate to anti-anxiety effectof probiotics and angiotensine receptor blocker in caffeine induced anxiety rat model. Methodology: The study was conducted in six groups (n=6). Caffeine was given intraperitoneally at a dosage of 25 mg/kg each day for 14 days. Blood samples, body temperature, and glucose measurements were taken prior to the trial. Caffeine was given intraperitoneally to G1-G5 mice at a dosage of 25 mg/kg. For groups 3 and 4, a probiotic formulation was given orally following the caffeine dosage. In group 5, a probiotic topical gel (30 mg) was given topically to the animals' foreheads. Diazepam (1 mg/kg) was given to group 2 animals. Results: The body weight was not changed significantly throughout the study period in all the groups. Mortality and clinical signs were not observed during study. When compared to the anxiety control group, there was no statistical significance in either of the groups' glucose levels. There was no statistical significance in Hemoglobin (Hb) levels of animals on day 14 when we compare with anxiety control group. The changes in SGOT and SGPT levels were not found to be statistically significant. There was no statistical significance in rectal temperature of animals. There was no statistical significance in all the groups in hot plate readings on day 7 when compared with anxiety control group. Whereas there was significance increase in latency time (time spent) was observed in group 2 & group 6 when compared to anxiety control group on day 14. Probiotic high dosage and probiotic topic gel treated animals similarly spent significantly more time in open arms than anxiety control animals (P< 0.01). High serotonin levels were observed In the anxiety control rats .however it reduced in treatment groups. Conclusion: The study concludes the potential benefit of probiotics and Losartan for the treatment of caffeine induced anxiety.