Impact of Diphenyl Diselenide on Acute Ethanol-Induced Disruption of Antioxidant Defense and Inflammatory Gene Expression in Rats

Abstract

Perturbation in antioxidant status which often leads to inflammation of liver cells and alteration in activities of purinergic enzymes are common outcomes of ethanol intoxication, this makes any compound that is able to prevent or repair the redox imbalances induced by alcohol intoxication a potential therapeutic measure. Hence, this study aims to assess the effect of diphenyl diselenide (DPDSe) on oxidative stress indicators, inflammatory genes and activities of purinergic enzymes in ethanol intoxicated male Wistar rats. 10mg/kg DPDSe was administered orally 30 minutes before and after a single dose of 13 ml/kg (28% ethanol solution) was given to the rats. Thereafter, the antioxidant status, activities of purinergic enzymes and expression of redox-sensitive genes in the rats were evaluated. It was observed that ethanol evoked high production of lipid peroxidation with concomitant decrease in thiol level, increased Nucleotidase and NTPDase activities, it also causes the downregulation of Nrf2 and upregulation of (NF-kB), however, pre- and post-treatment with DPDSe caused a reversal effects on the biochemical parameters and molecular parameters evaluated respectively. The reversal of ethanol-induced changes in biochemical parameters, expression of genes linked to inflammation and antioxidant status in the liver of acute ethanol intoxicated rats by DPDSe suggests that it has high prospect as a suitable therapeutic agent for hepatotoxicity linked with acute ethanol intoxication.