The Use of (O. Basilicum) Extract to Treat Litopenaeus vannamei Disease the Infected Bacteria Vibrio parahaemolyticus

Abstract

This research aims to treat vaname shrimp disease  infected with bacteriaVibrio parahaemolyticus. In this research, we used Completely Randomized Design (CRD) 4 treatments and 3 replications. The treatment being tested was the treatment of vaname shrimp disease by soaking in basil (O. basilicum) leaf extract with different concentrations, namely: Treatment A (0 mg/ml), treatment B (1.56 mg/ml), treatment C (3.12 mg/ml) and treatment D (12.5 mg/ml). The parameter observed was the number of bacterial coloniesV. parahaemolyticus maintenance media and in the hepatopancreas of vaname shrimp. Apart from that, observations were made on the survival rate of vaname shrimp and water quality parameters. The research results found that the best concentration in the treatment of diseaseV. parahaemolyticus in vaname shrimp at a concentration of 12.5 mg/ml with the lowest number of bacterial colonies in the rearing media, namely 1.20 x 102 CFU/ml. In the hepatopancreas of vaname shrimp, the lowest number of colonies was obtained at a treatment concentration of 12.5 mg/ml, namely 1.12 x 102 CFU/ml. The highest survival rate was obtained at a treatment concentration of 12.5 mg/ml of 86,67±5,77%. The range of water quality in research observations was obtained as follows salinity 28.72-29.05 ppt, temperature 26.71- 27.25OC, oxygen ranges from 5.72-7.81, pH 7.18-8.13 and ammonia 0.033-0.041ppm. Water quality during the research was in the optimal range for the life of vaname shrimp.