Skip to content
Research Article Open access CC BY 4.0

Determination of Lignin Modifying Enzymes from Pleurotus ostreatus and Lentinus squarrosulus

S. B. Chuku, E. O. Nwachukwu, I. O. Agbagwa, H. O. Stanley

Annual Research & Review in Biology · pp. 39–45 · Published 23 Jun 2021

10.9734/arrb/2021/v36i630388

Abstract

Fungi play vital roles as decomposers. White rot fungi are an eco-physiological group that degrades wood by the secretion of specialized extracellular enzymes including lignin-modifying enzymes. There is growing interest in the use of extracellular enzymes for bioremediation. This study determined the Lignin Modifying Enzymes (LMEs) associated with two species of mushroom, Pleurotus ostreatus and Lentinus squarrosulus. The qualitative study was conducted using agar medium substituted with chromogenic substances to determine the production of LMEs by the species. The appearance of colour change and clearance due to reaction with chromogenic substrates were used to determine LMEs production by the fungi. The results showed that Pleurotus ostreatus and Lentinus squarrosulus tested positive by the appearance of light brown colouration, reddish-brown colouration and discolouration of media for overall polyphenol oxidase, Laccase and Peroxidase activity, respectively. The study showed that the species studied are candidates for large scale production of LMEs that can be utilized as an eco-friendly solution for bioremediation of contaminated sites.

bioremediation laccase Lentinus squarrosulus lignin modifying enzymes Pleurotus ostreatus peroxidase

References (15)

  1. 1 Microbial enzymes: industrial progress in 21st century [DOI]
  2. 2 Mushrooms: Cultivation, Nutritional Value, Medicinal Effect, and Environmental Impact
  3. 3 A new assay for lignin-type peroxidases employing the dye azure B [DOI]
  4. 4 Screening for novel laccase-producing microbes [DOI]
  5. 5 Qualitative methods for the determination of lignocellulolytic enzyme production by tropical fungi
  6. 6 Cultivation Techniques and Medicinal Properties of Pleurotus spp.
  7. 7 Screening and assessment of laccase producing fungi isolated from different environmental samples [DOI]
  8. 8 Screening of some marine-derived fungal isolates for lignin degrading enzymes (LDEs) production.
  9. 9 Qualitative and Quantitative Methods for Isolation and Characterization of Lignin-Modifying Enzymes Secreted by Microorganisms [DOI]
  10. 10 "Lignocellulose Degrading Enzymes from Fungi and Their Industrial Applications" [DOI]
  11. 11 A lignolytic fungi with laccase activity isolated from Malaysian local environment for phytochemical transformation purposes
  12. 12 Tropical Soil Fungi Producing Cellulase and Related Enzymes in Biodegradation [DOI]
  13. 13 Growth Response of Pleurotus spp. on Different Basal Media and Different pH Levels
  14. 14 International Journal of Current Microbiology and Applied Sciences [DOI]
  15. 15 Agriculture and Biology Journal of North America [DOI]

Cited by 3

Research progress on the structure of edible fungi polysaccharides and their anti-obesity mechanisms via the regulation of intestinal microbiota and metabolites

Xin Wei, Weiya Rong, Liyang Jin · Food Bioscience · 2026

Evaluating the Efficacy of Biopulping on Poplar Wood Chips Using the White-rot Fungus Trametes SP.

M. Bisht, P. Meena · Ecology, environment & conservation · 2024

Showing 2 of 3 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

39

Page views

0

PDF downloads

4

Outbound clicks

3

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.