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Detection of Metastatic Lung Cancer by Immunocytochemistry and Flow Cytometry in a Sample of Pleural Fluid

Manoela Lira Reis, Rafaela Pilon, Aline Costa, Teresa Maria da Silva Figueiredo, Alisson Bigolin, Ana Carolina Rabello de Moraes, Renata Cristina Messores Rudolf de Oliveira, Maria Cláudia Santos da Silva

International Journal of Medical and Pharmaceutical Case Reports · pp. 1–5 · Published 11 Jun 2016

10.9734/IJMPCR/2016/26726

Abstract

Malignant pleural effusions (MPE) are common complications in cancer patients and indicate the spread of the primary disease (metastasis). For over a century, malignant effusion has been diagnosed through the observation of changes in the cavity effusion cells, such as the gold-standard cytomorphological analysis. Studies show that the multiparameter flow cytometric analysis is sensitive and rapid and allows for the immunophenotypic evaluation of a large number of cells. This report describes the case of a 64-year-old man diagnosed with lung cancer who had never had any kind of treatment‏ for this disease. The pleural sample was analyzed by morphological (quantitative and differential cytology) and immunocytochemical analyses. As a new tool for diagnosis of malignant pleural effusion, the sample was also analyzed by flow cytometry. In the case report described, flow cytometry was an effective and quick method for detecting neoplastic cells in pleural fluids. 

Malignant pleural effusions new tool for diagnosis flow cytometry.

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Cited by 1

Analysis of Cellular DNA Content in Pleural Effusion by Flow Cytometry During Lung Cancer Progression: A Case Report

Maria M Vasilescu, Marieta E Panait, Mirela Dumitru · Cureus · 2024

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