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Research Article Open access CC BY 3.0

Detection of FUS-1 (OXA-85), a Class D Beta-lactamase from Fusobacterium nucleatum Subspecies Polymorphum in Nigeria

Francisca O. Nwaokorie, Kofoworola O. Savage, Muinah A. Fowora, Patricia O. Ayanbadejo, Folasade T. Ogunsola, Akitoye O. Coker

Microbiology Research Journal International · pp. 492–500 · Published 25 Jul 2013

10.9734/BMRJ/2013/3599

Abstract

Aims: Beta-lactamase production and subsequent resistance to β-lactam drugs has been a global concern in the treatment of Gram negative anaerobes. The aim of this study was to identify F. nucleatum strains producing Class D β-lactamase through the detection of FUS-1 (OXA-85) resistance gene. Place and Duration of Study: Department of Preventive Dentistry, Lagos University Teaching Hospital, Idi-Araba, between February 2010 and November 2010. Methodology: Twenty two oral clinical samples were obtained from patients with chronic periodontitis who admitted to previous use of amoxicillin. Antibacterial susceptibility of the bacterial isolates was determined by E-test on Brucella Blood agar. Amplification of the bacterial DNA was carried out by PCR using F. nucleatum species-specific primer, FUS-1 specific for blaFUS-1 and strain-specific primers for subspecies nucleatum,, fusiforme, polymorphum and vincentii. Results: From the 19 samples collected, F. nucleatum was isolated, and the identity of the isolates was confirmed by PCR. Four of the isolates produced similar bands with the control strain, 3 (15.7%) strains were able to produce amplication with FUS-1 primer specific for blaFUS-1 gene found in β-lactamase producing F. nucleatum subsp. polymorphum. Conclusion: This study shows the presence of class D β-lactamase producing F. nucleatum species in Nigeria.

Beta-lactamase resistance genes Fusobacterium nucleatum subspecies polymorphum

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Cited by 3

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Francisca Nwaokorie, K. Okunade, U. Edet · Babcock University Medical Journal · 2023

Drug Resistance and Gene Transfer Mechanisms in Respiratory/Oral Bacteria

S. Jiang, Jumei Zeng, Xuedong Zhou · Journal of dentistry research · 2018

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