Detection of Antibiotic Resistance and Virulence Factors of Klebsiella Species

Abstract

Klebsiella species is a common cause of hospital acquired infections (HAIs) and more antibiotic resistance patterns seen in this species. The purpose of this study was to identify the antibiotic resistance such as ESBL producer,carbapenem- resistant K.pneumoniae (CRKP) and  study (Antibiogram) of Klebsiella species isolated from clinical samples in a tertiary care hospital in south Goa, India. The Klebsiella species was isolated from the hospital admitted patient process at Royal Hospital South Goa from April 2022 to Dec 2022. Identification of Klebsiella species isolate was done by analyzing microscopic examination, colony morphology, performing biochemical testing and also process on Vitek 2 compact system for confirming species identification and their susceptibility. A total of 213 (21.82 %) Klebsiella species isolates were isolated from 976 total clinical bacterial isolates during the study. In this study two different species of Klebsiella were studied such as Klebsiella pneumoniae(20.59%) and Klebsiella oxytoca (1.22%) . Most of them were collected from patients aged more than 45 years old. Klebsiella pneumoniae were obtained from respiratory specimens (38.49%). Among all (90%) isolates showed sensitivity to Meropenem, Amikacin, Piperacillin/tazobactam (80%) isolates showed sensitivity to Cefoperazone/sulbactam (60%) isolates showed sensitivity to Levofloxacin, (100%) isolates showed sensitivity to Tigecycline. Most common Klebsiella species present in hospital acquired infection is Klebsiella pneumoniae. Klebsiella pneumoniae isolates demonstrated resistance to a wide range of antibiotics.