Skip to content
Research Article Open access CC BY 4.0

Detection and Molecular Identification of Persistent Water Vessel Colonizing Bacteria in a Table Water Factory in Nigeria

O. Nwaiwu, M. I. Nwachukwu

Microbiology Research Journal International · pp. 1–12 · Published 16 Mar 2016

10.9734/BMRJ/2016/24378

Abstract

Aims: To establish organisms persistent in a table water production facility and to determine the points they entered in order to eliminate any risk of having pathogens in a water production system. Study Design: Analytic observational studies Place and Duration of Study: Bottling facility in Nigeria and University of Nottingham, United Kingdom. Study was between August 2011 and Sept 2013. Methodology: Sample control points were subjected to counts of bacteria, yeasts, and mold using membrane filtration to trace the source of increased bacteria counts in a table water production factory. Organisms were identified by 16S rRNA sequencing and biofilm formation was assessed with micro titer dish biofilm formation analysis. Results: Total bacteria and Pseudomonas Spp. count were highest in the carbon filter and 5% (v/v) chlorine used for disinfection was found to be effective against planktonic cells of 18 hour cultures of Pseudomonas Spp. isolated from the carbon filter tank. No yeasts and mold were detected and after a sand blasting exercise to clean affected tanks, total bacteria counts on tryptone glucose extract medium decreased to less than factory allowable limits of 25 cfu per 100 ml of water with no further growth of Pseudomonas Spp. on centrimide medium. Other bacteria that emerged after the sand blasting exercise were regarded as persistent but had less biofilm forming ability (p=.02) when compared with Pseudomonas aeruginosa PAO1. The persistent bacteria identified after sequencing were Aeromonas hydrophila and Serratia proteamaculans. Conclusion: Apart from Pseudomonas Spp., other bacteria can persist in the water tanks of a water bottling facility and routine checks may fail to detect an underlying problem until it becomes obvious. Prompt corrective action ensures that public safety is not compromised. This was the first time Aeromonas hydrophila and Serratia proteamaculans were identified from the water tanks in the bottling facility.

Persistence water vessel biofilms Aeromonas hydrophila Serratia proteamaculans Pseudomonas Spp

References (44)

  1. 1 Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA [DOI]
  2. 2 Bacterial biofilms: from the Natural environment to infectious diseases [DOI]
  3. 3 Biofilm Formation as Microbial Development [DOI]
  4. 4 Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development [DOI]
  5. 5 Microtiter Dish Biofilm Formation Assay [DOI]
  6. 6 Rapid extraction of bacterial genomic DNA with guanidium thiocyanate [DOI]
  7. 7 The Genus Aeromonas : Taxonomy, Pathogenicity, and Infection [DOI]
  8. 8 Concise review of mechanisms of bacterial adhesion to biomaterials and of techniques used in estimating bacteria-material interactions [DOI]
  9. 9 Food spoilage—interactions between food spoilage bacteria [DOI]
  10. 10 Resistance of bacterial biofilms to disinfectants: a review [DOI]
  11. 11 Biofilm formation and the food industry, a focus on the bacterial outer surface [DOI]
  12. 12 Microbial Agents Associated with Waterborne Diseases [DOI]
  13. 13 The Formation of Biofilms by Pseudomonas aeruginosa : A Review of the Natural and Synthetic Compounds Interfering with Control Mechanisms [DOI]
  14. 14 Risk Assessment of Pseudomonas aeruginosa in Water [DOI]
  15. 15 Microbial ecology of drinking water distribution systems [DOI]
  16. 16 Factors promoting survival of bacteria in chlorinated water supplies [DOI]
  17. 17 Microbiologically influenced corrosion of 304 stainless steel by aerobic Pseudomonas NCIMB 2021 bacteria: AFM and XPS study [DOI]
  18. 18 Influence of the Diversity of Bacterial Isolates from Drinking Water on Resistance of Biofilms to Disinfection [DOI]
  19. 19 Genetic control of bacterial biofilms [DOI]
  20. 20 Friend or foe? A review of the mechanisms that drive Serratia towards diverse lifestyles [DOI]
  21. 21 Management of dental unit waterline biofilms in the 21st century [DOI]
  22. 22 Bacteria attachment to surfaces – AFM force spectroscopy and physicochemical analyses [DOI]
  23. 23 Formation of natural biofilms during chlorine dioxide and u.v. disinfection in a public drinking water distribution system [DOI]
  24. 24 Bacterial community dynamics during the early stages of biofilm formation in a chlorinated experimental drinking water distribution system: implications for drinking water discolouration [DOI]
  25. 25 Growth of Escherichia coli in Model Distribution System Biofilms Exposed to Hypochlorous Acid or Monochloramine [DOI]
  26. 26 Antibacterial Effect of Water-Soluble Tea Extracts on Foodborne Pathogens in Laboratory Medium and in a Food Model [DOI]
  27. 27 Microbial contamination of dental unit waterlines.
  28. 28 Kinetics of biofilm formation and desiccation survival ofListeria monocytogenesin single and dual species biofilms withPseudomonas fluorescens,Serratia proteamaculansorShewanella balticaon food-grade stainless steel surfaces [DOI]
  29. 29 Microbiological influences in 'blue water' copper corrosion [DOI]
  30. 30 Performance of Pseudomonas CFC-selective medium in the fish storage ecosystems [DOI]
  31. 31 Biochemical and genetic characterization of arazyme, an extracellular metalloprotease produced from Serratia proteamaculans HY-3.
  32. 32 Antibiogram, Adhesive Characteristics, and Incidence of Class 1 Integron inAeromonasSpecies Isolated from Two South African Rivers [DOI]
  33. 33 Identification of Pseudomonas aeruginosa in water-bottling plants on the basis of procedures included in ISO 16266:2006 [DOI]
  34. 34 Seasonal variability of intestinal microbiota in rainbow trout (Oncorhynchus mykiss), with a particular attention to Aeromonas spp. as candidate indicator of antimicrobial resistance
  35. 35 Opportunistic pathogens and faecal indicators in drinking water associated biofilms in Cluj, Romania [DOI]
  36. 36 Molecular Detection, Quantification, and Toxigenicity Profiling of Aeromonas spp. in Source- and Drinking-Water [DOI]
  37. 37 Comparison of biofilm formation and water quality when water from different sources was stored in large commercial water storage tanks [DOI]
  38. 38 The relationship between microbial metabolic activity and biocorrosion of carbon steel [DOI]
  39. 39 Involvement of Organic Acid During Corrosion of Iron Coupon by Desulfovibrio desulfuricans
  40. 40 A multi-approach study of influence of growth temperature and nutrient deprivation in a strain of Aeromonas hydrophila [DOI]
  41. 41 Microbiologically induced corrosion of aluminum alloys in fuel-oil/aqueous system.
  42. 42 Psychrophilic trypsin-type protease from Serratia proteamaculans [DOI]
  43. 43 Development and Validation of a Real‐Time TaqMan Assay for the Detection and Enumeration of Pseudomonas fluorescens ATCC 13525 Used as a Challenge Organism in Testing of Food Equipments [DOI]
  44. 44 Microbial Biofilm as a Smart Material [DOI]

Cited by 9

Bacteriological Quality of Borehole and Sachet Water from a Community in Southeastern Nigeria

O. Nwaiwu, Chiugo Claret Aduba, Oluyemisi Eniola Oni · Pathogenic Bacteria · 2020

Optimization of ozone decomposition time and its effect on physicochemical and bacteriological quality of table water

O. Nwaiwu, V. Ibekwe · Croatian Journal of Food Science and Technology · 2019

Showing 7 of 9 known citations — external sources report more than can currently be individually listed.

Article metrics

Real usage data collected on this platform.

69

Page views

0

PDF downloads

6

Outbound clicks

9

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.