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Research Article Open access CC BY 3.0

Defects in Wistar Rat’s Embryo and Placenta Development: A [C]14-Morphine Study

Masoomeh Kazemi, Elaheh Tekieh, Sahar Golabi, Hedayat Sahraei

Annual Research & Review in Biology · pp. 2823–2834 · Published 7 May 2014

10.9734/ARRB/2014/9850

Abstract

Background: In previous studies it has been emphasized that morphine effectiveness site may be either in the embryo or the placenta. In this study, we perused the morphine effect site in embryo with placenta, simultaneously. Animals treated by C14-Morphine. Material and Methods: Experimental groups received daily doses of 0.05mg/ml C14-morphine in their drinking water. On the 9th and14th embryonic days, the rats were anesthetized, embryos with the placenta removed and were fixed in 10% formalin for two weeks. Then, processed, sectioned in 5 and 25µm thicknesses, and fixed on glass slides for further evaluation. The 25µm sections were delivered to black and white film for three days. Films processed and evaluated with a digital inverse microscope for possible radiological impression. The 5µm sections were processed for H&E staining, and evaluated by light microscope and MOTIC software. Results: Results showed that total surface of 9th- day placenta and embryo, increases simultaneously in compare with control one. 14th- day placenta, showed a significant decrease. Labeled morphine consumption data, revealed that maximum location of morphine effect aggregation is simultaneously on the placental villi and whole embryo. Conclusion: Morphine effect to detect embryo’s normal growth can be result from its effect on normal placental development and its passage from placental barrier.

Placenta development C14-morphine rat embryo development.

References (22)

  1. 1 A Comprehensive Review of Opioid-Induced Hyperalgesia [DOI]
  2. 2 Cellular and Synaptic Adaptations Mediating Opioid Dependence [DOI]
  3. 3 Endocrine mechanisms of intrauterine programming [DOI]
  4. 4 Programming placental nutrient transport capacity [DOI]
  5. 5 Effect of nutrient intake during pregnancy on fetal and placental growth and vascular development [DOI]
  6. 6 The Placenta and Intrauterine Programming [DOI]
  7. 7 The developmental outcome of children born to heroin-dependent mothers, raised at home or adopted [DOI]
  8. 8 Drug interactions at the blood-brain barrier: Fact or fantasy?☆ [DOI]
  9. 9 Animal models of placental angiogenesis [DOI]
  10. 10 Nutritionally Mediated Placental Growth Restriction in the Growing Adolescent: Consequences for the Fetus1 [DOI]
  11. 11 Drug transport across the placenta, role of the ABC drug efflux transporters [DOI]
  12. 12 Effects of maternal oral morphine consumption on neural tube development in Wistar rats [DOI]
  13. 13 The Effects of Cortisol on the Binucleate Cell Population in the Ovine Placenta During Late Gestation [DOI]
  14. 14 Attenuation by dextromethorphan on the higher liability to morphine-induced reward, caused by prenatal exposure of morphine in rat offspring [DOI]
  15. 15 The effects of morphine on cell proliferation [DOI]
  16. 16 Respiratory effects of chronic in utero methadone or morphine exposure in the neonatal guinea pig [DOI]
  17. 17 EFFECT OF ORAL MORPHINE CONSUMPTION IN FEMALE RATS ON DEVELOPMENT OF BRAIN CAVITIES, CENTRAL CANAL AND CHOROID PLEXUS OF THEIR EMBRYOS
  18. 18 Caffeine increases paragigantocellularis neuronal firing rate and induces withdrawal signs in morphine-dependent rats [DOI]
  19. 19 Morphine administration to pregnant rabbits: effect on fetal growth and lung development.
  20. 20 Identification of morphine accumulation in the rat embryo central nervous system: a c14-morphine administration study.
  21. 21 The Effect of Oral Morphine Consumption on Ependymal Duct and Spinal Cord Development in Wistar Rats Embryos
  22. 22 Clinical and Developmental Immunology [DOI]

Cited by 1

Embryonic Central Nervous System Development under Morphine Influence in Pregnant Rats as the Epigenetic Factor

M. Kazemi · 2014

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