Cytotoxicity of Eurycomanone and Fargesin in RAW 264.7 Murine Macrophages
Hanim Akmar Rosly, Amira Nabila Mat Roof, Salfarina Ramli, Visarut Buranasudja, Pornchai Rojsitthisak, Boonchoo Sritularak, Hasseri Halim
Journal of Pharmaceutical Research International · pp. 275–284 · Published 18 Aug 2021
10.9734/jpri/2021/v33i41A32327Abstract
Bioactive natural compounds derived from plants are the source for the development of new drugs. Numerous in vitro studies have explored the anti-inflammatory effect of eurycomanone and fargesin, derived from Eurycoma longifolia and Flosmagnoliae, respectively. However, before anti-inflammatory investigation is conducted, it is important to obtain the safe doses of these compounds to ensure the validity of the anti-inflammatory results. Therefore, the present study was aimed to investigate the cytotoxicity of eurycomanone and fargesin towards macrophage RAW 264.7. cells to determine the safe doses of these compounds. Different concentrations of eurycomanone and fargesin were subjected to RAW 264.7 cells. The cytotoxicity of the compounds was evaluated by MTT assay and 50% inhibitory concentration (IC50) of these compounds was determined. Morphological changes of RAW 264.7 cells upon exposure to these compounds were also observed. Eurycomanone exhibited its cytotoxic effect by reducing RAW 264.7 cell viability dose-dependently with the IC50 of 94.17 µM. Meanwhile, fargesin had slight cytotoxicity towards RAW 264.7 cells with the IC50 of 173.5µM. Eurycomanone was more cytotoxic towards RAW 264.7 cells compared to fargesin. In conclusion, eurycomanone and fargesin at concentration up to 25 µM, was not toxic to the RAW 264.7 murine macrophages cells and the findings can be applied in the future anti-inflammatory study.
Cited by 1
1 citation reported by external sources — individual citing-article records aren't available to list yet.
Related research
- In vitro Cytotoxicity and Antiplasmodial Activity of Fractions from Anthocleista djalonensis A. Chev. Acetone Root Extract — shares topic coverage
- Anti-microbial Activities of Selected Ghanaian Medicinal Plants and Four Structurally Similar Anti-protozoan Compounds against Susceptible and Multi-drug Resistant Bacteria — shares topic coverage
- Comparison of the Antioxidant Activity of Aromatic Medicinal Plants of Moroccan Origin — shares topic coverage
- Study of Antioxidant Activity of Essential Oils Extracted from Moroccan Medicinal and Aromatic Plants — shares topic coverage
- Anti-proliferative Activity of Prunus africana, Warburgia stuhlmannii and Maytenus senegalensis Extracts in Breast and Colon Cancer Cell Lines — shares topic coverage
Article metrics
Real usage data collected on this platform.
2
Page views
0
PDF downloads
0
Outbound clicks
1
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.