Comparative In-vitro Antioxidant and Antimicrobial Activity of Hydroalcoholic Extract of Miliusa tomentosa

Abstract

Green medicine, the drugs derived from plants attained a wide spread interest, as believed to be safe and dependable, compared with costly synthetic drugs that have adverse effects. The present study aimed to analyze a comparative in vitro free radical scavenging and antimicrobial potentials of leaf hydroalcoholic extract fractions of Miliusatomentosa (Roxb.) J. Sinclair (M. tomentosa, Annonaceae) commonly known as hoom, kari.It is a large deciduous tree, growing up to 20 m tall and are given to children to cure the weakness in summer. The hydroalcoholicextract of leaves of M. tomentosa was studied for antioxidant activity on different in vitro models namely 1,1-diphenyl, 2-picryl hydrazyl (DPPH) assay, Hydrogen peroxide (H2O2) and Nitric oxide (NO) radical scavenging method. Agar well diffusion method has been used to determine the antimicrobial activities of plant extracts against Gram-positive bacteria, Gram-negative bacteria (Enterococcus faecalis, Salmonella Bongori,Propionibacterium acnes), and fungus (Aspergillusflavus). In the investigated models, the extract showed dose-dependent free radical scavenging properties. For the DPPH technique, M. tomentosa leaves extract had an IC50 value of 335.92g/ml, which was close to that of ascorbic acid (IC50=17.68g/ml). The IC50 value for hydrogen peroxide was determined to be 96.42g/ml, which compares favourably to ascorbic acid (IC50=36.61g/ml). The IC50 value for nitric oxide was 105.87g/ml, which was close to that of ascorbic acid (IC50=24.63g/ml). The extracts exhibited both antibacterial and antifungal activities against tested microorganisms using standardampicilin, ciprofloxacin, clindamycin and fluconazole (10-30μg/ml). The antimicrobial activity was determined by measuring the diameter of the zone of inhibition in term of millimeter (mm). The antimicrobial activity of hydroalcoholic extract of leaves against all microorganisms was concentration dependent but less than standard drug. On the basis of results obtained, it is suggested that both M. tomentosa leaves extracts may be a potential source of natural antioxidants and antimicrobial compounds to be used in the treatment of various oxidative disorders, infectious diseases caused by resistant microorganisms.