Skip to content
Research Article Open access CC BY 4.0

Comparative Evaluation of the Antibacterial Effects of Nigella sativa and Moringa oleifera on some Clinical Bacteria Isolates

D. E. Garba, M. U. E Dibua, F. Attah, E. C. Alechenu

Journal of Advances in Microbiology · pp. 7–19 · Published 26 Mar 2021

10.9734/jamb/2021/v21i330331

Abstract

Aim: In this study, the antibacterial effect of the n-hexane, methanol, and ethanol extracts of two traditional medicinal plants, Nigella sativa and Moringa oleifera was carried out to comparatively assess the antibacterial efficacy of these plants against some selected multi-drug resistant diarrheagenic bacterial isolates such as Escherichia coli, Salmonella typhi, Shigella spp, Enterobacter aerogenes, Klebsiella pneumonia, and Pseudomonas aeruginosa. Methodology: The inhibitory effects of extracts were assessed using disk diffusion method at six different concentrations i.e. 12.5 mg/ml, 25 mg/ml, 50 mg/ml, 100 mg/ml, 200 mg/ml, and 400 mg/ml. The minimum inhibitory concentrations and minimum bactericidal concentrations of the plant seed extracts were determined by the micro-broth dilution technique. Synergism and antagonism studies were similarly done to determine the combined effects of both N. sativa and M. oleifera seed oil. Further, resistant bacteria were subjected to sodium dodecyl sulphate treatment for plasmid curing and continued with the antibiotic susceptibility test. Results: The oil extracts of N. sativa were found to be more effective than that of M. oleifera (at MIC and MCC of 50 mg/ml and 100 mg/ml respectively). It was observed that the plasmids were cured in all the E. coli isolates. Among the pathogens, E. coli was highly susceptible to both seed oils. Conclusion: Higher antibacterial activity was observed when the extracts were used in combination, showing a strong synergistic effect against all the pathogens tested.

Nigella sativa Moringa oleifera antimicrobial agents plasmid curing multi-drug resist ance antibacterial

References (23)

  1. 1 National Committee for Clinical Laboratory Standards [DOI]
  2. 2 Glossary of Indian Medicinal Plants
  3. 3 Antimicrobial activity of essential oils and other plant extracts [DOI]
  4. 4 Moringa oleifera : a food plant with multiple medicinal uses [DOI]
  5. 5 Compendium of Indian medicinal plants
  6. 6 Antimicrobial resistance in bacteria isolated from aquaculture sources in Australia [DOI]
  7. 7 Studies on phytochemical constituents of six Malaysian medicinal plants [DOI]
  8. 8 Laboratory Exercises in Microbiology
  9. 9 Laboratory Exercises in Microbiology
  10. 10 Antimicrobial Activity of Plant Secondary Metabolites: A Review [DOI]
  11. 11 Antibacterial effects of Balanites aegyptiaca L. Drel. and Moringa oleifera Lam. on Salmonella typhi [DOI]
  12. 12 Antibacterial Activity of Leaf Juice and Extracts of Moringa oleifera Lam. against Some Human Pathogenic Bacteria
  13. 13 Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.) [DOI]
  14. 14 Screening of Methanol and Acetone Extracts of Fourteen Indian Medicinal Plants for Antimicrobial Activity
  15. 15 Antibacterial activity of Moringa oleifera and Moringa stenopetala methanol and n-hexane seed extracts on bacteria implicated in water borne diseases [DOI]
  16. 16 Isolation, analysis and identification of phytochemicals of antimicrobial activity of Moringa oleifera Lam.
  17. 17 Widespread plasmid resistance genes among Proteus species in diabetic wounds of patients in the Ahmadu Bello university teaching hospital (ABUTH) Zaria [DOI]
  18. 18 Extraction of Essential Oil from Nigella sativa Using Supercritical Carbon Dioxide: Study of Antibacterial Activity [DOI]
  19. 19 Pharmacological and pharmaceutical potential of Moringa oleifera: a review.
  20. 20 Antioxidant and antibacterial activities of polyphenolic compounds from bitter cumin (Cuminum nigrum L.) [DOI]
  21. 21 Antibiotic Resistance Patterns of Staphylococcus aureus Isolated from Nostrils of Healthy Human Subjects in a Southeastern Nigeria Locality
  22. 22 African Journal of Microbiology Research [DOI]
  23. 23 Indian Journal of Applied Microbiology [DOI]

Cited by 2

Comparative Evaluation of Tensile Strength and Knot Strength of Nigella sativa Coated and Non-Coated Sutures: An In vitro Study

K. Vennila, S. Shakeron, G. R. Shangeetha · International Journal of Oral Care and Research · 2025

Showing 1 of 2 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

5

Page views

0

PDF downloads

0

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.