Co-relative Study of Serum Prolidase Activity and Oxidative Status in Patients with Diabetic Neuropathy

Abstract

Objective: Diabetic neuropathy is one of the most common long term complication of diabetes. Associaton of oxidative stress have been reported in diabetes. The study was aimed to evaluate oxidative status in diabetic neuropathy patients by estimation of serum total oxidative status (TOS), total antioxidant status (TAS) and prolidase activity. Methods: Sixty (60) patients of diabetic neuropathy were included in the study and 60 normal subjects were taken as control. TOS and TAS were measured in serum extract by colometric test. Along with these laboratory parameters, demographic and neurological examination was also done. Results: The activity of TOS, TAS, oxidative stress index (OSI) and serum prolidase activity were higher in cases as compared to control.TOS (p<.0001) has a weak positive correlation with very low density lipoprotein (VLDL). TAS (p>.05) is negatively correlated with basal metabolic index, serum triglyceride (STG), serum cholesterol (SC), very low density lipoprotein (VLDL), high density lipoprotein (HDL), fasting blood sugar (FBS), post prandial blood sugar (PPBS) and HbA1c. In our study prolidase activity was elevated in cases as compared to control that was not significantly different (P>.001). A significant positive correlation with HDL (r= .418**) and moderate negative correlation with HbA1C (r = -.318*), VLDL (r = -.308*), STG (r = -.301*) was found. Conclusion: Thus, our study concludes that TOS and OSI have a significant role in diabetic neuropathy. In addition to this increased serum prolidase activity in diabetic neuropathy patients may be due to increased collagen turnover. Increase in TAS activity needs to be further investigated as the sample size of this study was small and although it was an accidental finding.