Frequency of ABCB1 Polymorphisms in Breast Cancer Patients in South Egypt Cancer Institute by High-Resolution Melting Curve Analysis (Pilot Study)

Abstract

Introduction and Aim of the Work: The ABC (ATP-binding cassette) transporter family contributes to the multidrug resistance the genetic variants of this gene have been intensively studied so far.  Some single nucleotide polymorphism (SNPs) of ABCB1 has a correlation with the risk of breast cancer. So this study was conducted to assess value of using of 2 SNPs gene as predicator of risk in Egyptian breast. Study Design: It is a pilot study to evaluate the value of using 2 SNPs gene as predicator of risk in Egyptian breast. Patients and Methods: Between September 2016-2017, 50 female breast cancer patients and 20 healthy females control group were included. Peripheral blood samples from patients and control were taken for genomic DNA extraction. And HRM experiment was designed to assess (rs2214102) and (rs2032582) (single nucleotide polymorphism) SNPs of ABCB1 gene this in South Egypt Cancer Institute. Results: Regarding (rs2214102), all samples have the same heterozygous genotype (GA) with no significant difference between cases and controls. On the other hand, samples of (rs2032582) revealed that samples are divided into 3 groups one is wild homozygous genotype (GG) and other 2 homozygous variants (TT) and (AA). The frequency of the 3 variants in patients and control group showed no statistical difference P=.057. Conclusion: Using SNPs rs2214102 and rs2032582 are not good predictive factors for breast cancer risk or development.