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Research Article Open access CC BY 3.0

Bacterial Colonization of Mechanical Ventilation Circuits in a Pediatric Intensive Care Unit

Daniela G. Moreira, Eitan Naaman Berezin, Marcelo Jenne Mimica

Microbiology Research Journal International · pp. 598–606 · Published 18 Feb 2014

10.9734/BMRJ/2014/6153

Abstract

To identify the onset time of bacterial proliferation in mechanical ventilator circuits in a pediatric intensive care unit (PICU) and the colonizing agents involved, as well to verify any possible pre-use contamination, we conducted a prospective microbiological analysis of the circuits of 30 patients, with a total of 342 cultures. Bacterial colonization of the mechanical ventilator circuits in the studied PICU was confirmed. Microorganisms were present in 39% of expiratory branch cultures: K. pneumoniae (77%), A. baumannii (65%), and S. aureus (42%). The inspiratory branch registered a smaller number of positive culture: A. baumannii (51%), S. aureus, coagulase-negative (38%) and K. pneumoniae (32%). As to the colonization onset time, 23% of the cultures collected on the 1st day from the inspiratory branch were positive, versus 30% of those collected from the expiratory branch, which lead us to conclude that bacteria can be present within the first hours of use. The sequential analysis of bacterial colonization over the course of circuit usage and the observation that the number of positive cultures becomes progressively higher on the 5th, 6th and 7th days in the expiratory branch both suggest that the systems should be replaced more often.

Bacterial infections mechanical ventilation pediatric intensive care

References (10)

  1. 1 Manual of Clinical Microbiology [DOI]
  2. 2 Effect of Ventilator Circuit Changes on Ventilator-Associated Pneumonia: A Systematic Review and Meta-analysis [DOI]
  3. 3 Management and prevention of ventilator-associated pneumonia caused by multidrug-resistant pathogens [DOI]
  4. 4 Nosocomial Infections and Multidrug-Resistant Bacterial Organisms in the Pediatric Intensive Care Unit [DOI]
  5. 5 Healthcare-associated infection prevention in pediatric intensive care units: a review [DOI]
  6. 6 Prevention of healthcare-associated infections in children: new strategies and success stories [DOI]
  7. 7 Contamination of Mechanical Ventilators with Tubing Changes Every 24 or 48 Hours [DOI]
  8. 8 Conhecimento dos profissionais de saúde na Unidade de Terapia Intensiva sobre prevenção de pneumonia associada à ventilação mecânica [DOI]
  9. 9 Evaluation of prevention and control measures for ventilator-associated pneumonia [DOI]
  10. 10 Manual of Clinical Microbiology. [DOI]

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