Bacterial Analysis for Seminal Fluid befor In-vitro Fertilization Procedure
S. O. Noor, A. Albalawi, H. Abduljabbar, Ebtesam H. Alosaimi, Sabah M. Hassan, A. Najjar
Journal of Pharmaceutical Research International · pp. 85–92 · Published 29 Sep 2020
10.9734/jpri/2020/v32i2330793Abstract
In vitro fertilization (IVF) is the most advanced technique of assisted reproductive where the sperm reach the eggs for fertilization in a laboratory tube. Seminal fluid often contains bacterial contaminants that decrease the quality and quantity of seminal fluids. Aim: In this study we investigated the presence of bacteria in semen before IVF procedure. Methodology: All samples were identified according to the morphological, biochemical, molecular, and antimicrobial tests. Results: Microbial species were detected in 28% of semen samples, while 72% did not show any bacterial growth. The prevalent bacterial isolates were Staphylococcus spp., Enterococcus spp., Catellicoccus spp., and Pseudomonas spp. Conclusion: The use of microbiological analysis to identify resistant bacterial isolates is considered an effective method to reduce infertility in men by changing the contaminated semen.
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