Assessment of the Woundy Healing Activity of the Aqueous Extract of Leaves of Diospyros hoyleana (F.) White (Ebenaceae)

Abstract

Wounds are an extremely common reason for consultation in emergency departments. They mostly result from traffic accidents but also of different traumatisms. Their healing is possible thanks to a natural, spontaneous and coordinated process set up by the body. This process can be accelerated by the use of synthetic, biosynthetic or natural healing agents. Indeed, many medicinal plants are endowed with healing properties such as Diospyros hoyleana F. White, whose leaves help in the management of wounds. The present study consists in carrying out a phytochemical screening, evaluating the acute toxicity (oral and cutaneous) and determining the healing activity of the aqueous extract of Diospyros hoyleana leaves in comparison with that of BIAFINE®. A phytochemical screening was carried out in order to highlight the presence of secondary metabolites responsible for the healing activity. Acute oral and cutaneous toxicity tests were carried out on different batches of rats. Subsequently, the evaluation of the healing activity by induction of traumatic lesions on batches of rats was made. The evolution of wounds of the different batches was compared on the one hand by calibrated photography and on the other hand by histological analysis. At the end of this study, the results show that the aqueous extract of Diospyros hoyleana leaves has a healing activity with a healing profile at doses of 100 and 200 mg/kg similar to that of BIAFINE. The aqueous extract of Diospyros hoyleana leaves has healing activity with a healing profile similar to that of trolamine at doses of 100 mg/kg and 200 mg/kg. Flavonoids or tannins could be responsible for this activity.