Assessment of Antisporulant Activities of Wild Mushroom against Phytopathogenic Fungi

Abstract

Fruit body extract of twenty wild mushrooms growing across Kangra and Mandi district of Himachal Pradesh, India were collected and has been evaluated for antisporulant activity against Alternaria brassicae, Rhynchosporium oryzae, Fusarium oxysporum f.sp. psi and Colletotrichum capsici. Initial screening was conducted at 50% concentration of fruit body extracts to identify potential mushroom extracts. Seventeen wild mushrooms extract exhibit varying degree of antisporulant activity which ranged from 0 to 100 % inhibition depending upon fungal pathogen. Complete spore germination of Rhynchosporium oryzae was found to be inhibited by Lactarius sp. and Coprinus sp. followed by 96.25, 95.00 and 91.66 per cent against Alternaria brassicae with Phellinus sp., Amanita sp., and Rusula sp. respectively. On the basis of per cent inhibition in spore germination, wild mushrooms have categorized into six group. Strongly active inhibition (91-100 %) was observed in Alternaria brassicae and Rhynchosporium oryzae followed by very active inhibition (76-90 %) in Rhynchosporium oryzae. However, active (50-75%) and moderately active inhibition (20-49 %) were found among all the tested phytopathogens. To determine minimal inhibitory concentration (MIC) of potential mushroom extracts was further screened at 10, 20, 25, 50 and 80 per cent concentration over phytopathogenic fungi. MIC at 10% was found to be in fruit body extract of Phellinus sp., Amanita sp., Ganoderma lucidum over Alternaria brassicae followed by Lactarious sp., Russula sp., and Amanita sp. in Rhynchosporium oryzae and Fusarium oxysporum.