Skip to content
Research Article Open access CC BY 4.0

Ascertainment of In vivo Antidiarrheal and In vitro Thrombolytic Effect of Ethanolic Extract of Leaves of Amomum dealbatum

Md. Azimul Islam, Mohammed Aktar Sayeed, Md. Abdul Barek, Enama Nabi Shetu, Md. Nurul Faisal

Journal of Applied Life Sciences International · pp. 1–8 · Published 25 May 2019

10.9734/jalsi/2019/v21i130093

Abstract

Aims: The present study aimed to investigate antidiarrheal and thrombolytic effect of ethanolic extract of leaves of A. dealbatum in mice. Study design: Antidiarrheal effect was evaluated by castor oil-induced diarrhea method at two different concentrations in mice and in vitro thrombolytic activity was analyzed with clot lysis assay of human blood. Place and duration of study: Department of Pharmacy, International Islamic University Chittagong, Kumira, Chittagong-4318, Bangladesh, between December 2018 and February 2019. Methodology: The male Swiss mice’s were divided into four groups (n = 5). First group was orally treated with 1% Tween-80 (10 ml/kg) and second group was orally treated with loperamide (5 mg/kg). Third and fourth group were orally treated with ethanolic extract of leaves of A. dealbatum at 200 and 400 mg/kg accordingly. Human RBCs were collected for conducting thrombolytic assay. During this study, 1.5 ml of venous blood was drawn from healthy volunteers (n = 10) and Streptokinase was employed as positive control and distilled water was employed as negative control. Results: In castor oil induced diarrhea model, ethanolic extract of leaves of A. dealbatum at 200, 400 mg/kg and loperamide (5 mg/kg) significantly reduced the number of feces and increase percent of inhibition of defecations compared to negative control. The extract showed percent of inhibition of defecation of 16.67 and 37.50 for 200 and 400 mg/ml respectively where the positive control loperamide showed 66.67%. Percentage of clot disruptions were 4.51 (p<.001), 75.69 (p<.001) and 26.07 (p<.001) for water, streptokinase and 10 mg/ml extract respectively. Conclusion: Based on the results from in vivo and in vitro activities, the leaves of A. dealbatum were found to be a potential source of new antidiarrheal and thrombolytic agents.

Amomum dealbatum anti-diarrheal castor oil thrombolytic clot disruptions

References (21)

  1. 1 Medicinal plants and traditional medicine in Africa
  2. 2 Overview of the coagulation system [DOI]
  3. 3 Paleobotany: The Biology and Evolution of Fossil Plants
  4. 4 Development of an in vitro model to study clot lysis activity of thrombolytic drugs [DOI]
  5. 5 Inhibition of Intestinal Motility and Secretion by Flavonoids in Mice and Rats: Structure-activity Relationships [DOI]
  6. 6 Studies on the antidiarrhoeal activity of Aegle marmelos unripe fruit: Validating its traditional usage [DOI]
  7. 7 Studies on anti-diarrhoeal activity of Calotropis gigantea R.Br. in experimental animals.
  8. 8 Nitric oxide and castor oil-induced diarrhea. [DOI]
  9. 9 Antidiarrhoeal and antimicrobial activity of Calpurnia aurea leaf extract [DOI]
  10. 10 De-novo portal vein thrombosis in liver cirrhosis [DOI]
  11. 11 Effects of organic extracts of six Bangladeshi plants on in vitro thrombolysis and cytotoxicity [DOI]
  12. 12 Evaluation of the anti-diarrheal activity of the leaf extract of Croton macrostachyus Hocsht. ex Del. (Euphorbiaceae) in mice model [DOI]
  13. 13 Analgesic and antidiarrheal properties of Ocimum selloi essential oil in mice [DOI]
  14. 14 Ricinoleic acid stimulation of active anion secretion in colonic mucosa of the rat. [DOI]
  15. 15 Antidiarrhoeal Activity of an Ethanol Extract of the Stem Bark of Piliostigma reticulatum (Caesalpiniaceae) in Rats [DOI]
  16. 16 Antioxidant, antidiarrheal, hypoglycemic and thrombolytic activities of organic and aqueous extracts of Hopea odorata leaves and in silico PASS prediction of its isolated compounds [DOI]
  17. 17 Time Course of PAF Formation by Gastrointestinal Tissue in Rats After Castor Oil Challenge [DOI]
  18. 18 Investigation of in vitro thrombolytic potential of ethanolic extract of Momordica charantia fruits: An anti-diabetic medicinal plant
  19. 19 ETHICAL CONSIDERATIONS IN THE USE AND CARE OF LABORATORY ANIMALS [DOI]
  20. 20 Cytotoxic and antioxidant activities of two species of ginger extracts
  21. 21 Streptokinase is more economical than alteplase [DOI]

Cited by 2

Identification of Antioxidant Compounds using the DPPH Radical Scavenging Method from Ethanol and Methanol Extracts of the Leaves and Fruits of the Renggak Plant (Ammomum dealbatum R.)

Rahmawati Rahmawati, M. Meliana, Lestia Furnapasta · ALCHEMY Jurnal Penelitian Kimia · 2026

Volatile Constituents of Amomum argyrophyllum Ridl. and Amomum dealbatum Roxb. and Their Antioxidant, Tyrosinase and Cytotoxic Activities

Aknarin Pintatum, S. Laphookhieo · Arabian Journal of Chemistry · 2022

Related research

Article metrics

Real usage data collected on this platform.

60

Page views

0

PDF downloads

69

Outbound clicks

2

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.