Antioxidant Potential of Extra Polymeric Substances From Marine Actinobacteria Streptomyces Species

Abstract

Introduction: The Actinobacteria are a phylum of Gram-positive bacteria. They can be terrestrial or aquatic. They are of great economic importance to humans because agriculture and forests depend on their contributions to soil systems. They are more abundant in soils than other media, especially in alkaline soils and soils rich in organic matter, where they constitute an important part of the microbial population. Material and Methods: The sediment sample was collected from the Gulf of Mannar, Tamilnadu. The collected sample was sun dried for 48 hrs and turned into fine powder by mortar and pestle. The colour of the mature sporulating aerial mycelium was recorded in naked eye. Results: The antioxidant potential of actinobacterial EPS was determined on the basis of their scavenging activity of the stable 1,1- diphenyl-2-picryl hydrazyl (DPPH) free radical. Different concentrations (0.5-3mg/ml) of samples were mixed with 2.9ml diphenylpicrylhydrazyl (DPPH) solution (120µM) in methanol and incubated in darkness at 37°c for 30 minutes. The absorbance was recorded at 517 nm. Data were implied as mean ± SEM. Conclusion: The produced melanin pigment from the Actinobacteria of streptomyces species was found to have potent antioxidant potential activity. Further characterisation and bio active properties should be done in the further studies, and more articles in future are yet to come in various properties of melanin pigment.