Antioxidant, Cytotoxicity and In vitro Anti-inflammatory Study on the Different Extracts of Stems and Roots of the Two Medicinal Plants: Cassia sophera and Jatropha curcas

Abstract

Different extracts of stems and roots of Cassia sophera and Jatropha curcas were screened for antioxidant, cytotoxic activity and in-vitro anti-inflammatory activity using various methods. DPPH free radical scavenging assay of Cassia sophera & Jatropha curcas showed that methanol extract of root and stem have significant antioxidant activity with IC50 value 2.42 & 7.49 µg/ml, respectively in comparison to standard with IC50 value 4.38 µg/ml. In total phenolic content determination experiment, the results showed that, for cassia sophera, methanol extract of stems have higher phenolic content i.e 273.14±10.69 mg/g as equivalent to gallic acid whereas for Jatropha curcas it is ethyl acetate extract of stem with value 154.53±5.76 mg/g. The total flavonoids content study of the extracts of Cassia sophera exhibited that, ethyl acetate extract of root has higher flavonoid content i.e. 105.37±5.89 mg/g of flavonoids as equivalent to quercetin whereas for Jatropha curcas it is chloroform extract of stem with value 50.28±6.55 mg/g. The cytotoxicity study exhibited that all the extracts have lower cytotoxic property compared to standard for Cassia sophera whereas for Jatropha curcas, chloroform extract of stem has significant cytotoxicity with LC50 value 4.52 μg/ml compared to standard vincristine sulfate with LC50 value 3.04 µg/ml. From in-vitro anti-inflammatory assay of methanol extracts of stems and roots, the result showed that both plants have strong activity in compared to standard diclofenac sodium.