Skip to content
Research Article Open access CC BY 3.0

Antinociceptive and Anti-inflammatory Activity of the Bark Extract of Plumeria rubra on Laboratory Animals

Banibrata Das, Tarana Ferdous, Qazi Asif Mahmood, J. M.A. Hannan, Rajib Bhattacharjee, Biplab Kumar Das

European Journal of Medicinal Plants · pp. 114–126 · Published 2 Feb 2013

10.9734/EJMP/2013/1026

Abstract

Aims: To evaluate the analgesic and anti-inflammatory effects of ethanolic bark extract of Plumeria rubra on experimental animal models. Study Design:  Assessment of antinociceptive and anti-inflammatory activity. Place and Duration of Study: Department of Pharmacy, North South University, Dhaka, Bangladesh, between January 2011 and June 2011. Methodology: The analgesic activity was evaluated by hot plate, acetic acid induced writhing and formalin induced writhing method in Swiss Albino mice divided into 4 different groups (control, standard diclofenac sodium and extract at two different doses of 250 and 500 mg/kg BW). The extract was also investigated for the anti-inflammatory effect on Long Evans rats using carrageenan induced rat paw edema method. For anti-inflammatory study, 24 rats were divided into 4 different groups each receiving either distilled water, standard drug or the extract at the doses of 250 and 500 mg/kg BW. Results: Phytochemical analysis of the extract revealed the presence of tannins, alkaloids, flavonoids and terpenoids. The extract elicited a highly significant (p<0.001) analgesic activity in a dose dependent manner on hot plate method, acetic acid induced writhing test and also on both the early and late phases of formalin test at the doses employed. In the hot plate method, the extract increased the reaction time of heat sensation to 60.81% and 66.52% at the doses of 250 and 500 mg/kg BW respectively while that of the standard drug was 57.40% at the 3rd hour of study. In acetic acid induced writhing test, the percent inhibition of writhing response by the extract was 62.87% and 70.66% at 250 and 500 mg/kg doses respectively (p<0.001) which were even better than the standard drug diclofenac sodium (50.30%). The extract also significantly inhibited the licking response at the dose of 500 mg/kg in both the early phase (55.11%, p<0.01) and the late phase (66.43%, p<0.01) of formalin test while the standard drug inhibited by 52.27% and 72.03%, respectively. The oral administration of the extract significantly (p<0.001) inhibited inflammatory response induced by carrageenan in a dose dependent fashion. The most prominent inhibition of 61.68% (250 mg/kg) and 73.65% (500 mg/kg) were observed at the 4th hour of study. Conclusion: The central and peripheral analgesic as well as anti-inflammatory effect of the ethanolic bark extract of P. rubra may be due to the presence of various chemical constituents specially flavonoids, tannins, alkaloids or terpenoids. These experimental findings would further establish the scientific basis of the traditional uses of the plant in the management and/or control of pain as well as inflammatory conditions.  

Plumeria rubra medicinal plant phytochemical screening analgesic anti-inflammatory acetic acid formalin carrageenan

References (52)

  1. 1 Carrageenin-Induced Edema in Hind Paw of the Rat as an Assay for Antiinflammatory Drugs [DOI]
  2. 2 Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis
  3. 3 ACETIC ACID FOR ANALGESIC SCREENING
  4. 4 Phytochemical Methods. A Guide to Modern Techniques of Plant Analysis. [DOI]
  5. 5 Inflammation 2010: New Adventures of an Old Flame [DOI]
  6. 6 Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms [DOI]
  7. 7 Modified formalin test: characteristic biphasic pain response [DOI]
  8. 8 BIPHASIC DEVELOPMENT OF CARRAGEENIN EDEMA IN RATS [DOI]
  9. 9 Nonsteroidal Antiinflammatory Drugs — Differences and Similarities [DOI]
  10. 10 Release of prostaglandins E and F in an algogenic reaction and its inhibition [DOI]
  11. 11 Effect of Plant Flavonoids on Immune and Inflammatory Cell Function [DOI]
  12. 12 Biological Properties of Citrus Flavonoids Pertaining to Cancer and Inflammation [DOI]
  13. 13 Naturally occurring antinociceptive substances from plants [DOI]
  14. 14 Participation of the sympathetic system in acetic acid-induced writhing in mice.
  15. 15 Phytochemical constituent and antioxidant activity of extract from the leaves of Ocimum gratissimum [DOI]
  16. 16 Screening of the topical anti-inflammatory activity of some Central American plants [DOI]
  17. 17 Anti-inflammatory, anticholinesterase, antioxidant and phytochemical properties of medicinal plants used for pain-related ailments in South Africa [DOI]
  18. 18 Immunomodulatory and Anti-inflammatory Effects of Semecarpus anacardium LINN. Nut Milk Extract in Experimental Inflammatory Conditions [DOI]
  19. 19 Acute and chronic antiinflammatory effects of plant flavonoids [DOI]
  20. 20 Alpha9 nicotinic acetylcholine receptors and the treatment of pain [DOI]
  21. 21 Antinociceptive, anti-inflammatory, and antipyretic properties of an aqueous extract of Dicranopteris linearis leaves in experimental animal models [DOI]
  22. 22 Antinociceptive and anti-inflammatory activity of a flavonoid isolated from Caralluma attenuata [DOI]
  23. 23 Flavonoids with anti-inflammatory and antinociceptive activity from Cistus laurifolius L. leaves through bioassay-guided procedures [DOI]
  24. 24 Anti-inflammatory activities of the methanol extracts and an isolated furanoditerpene constituent of Sphenocentrum jollyanum Pierre (Menispermaceae) [DOI]
  25. 25 Analgesic and anti-inflammatory activities of ethanol root extract of Mahonia oiwakensis in mice [DOI]
  26. 26 STUDIES ON THE ANTI-INFLAMMATORY AND ANTI-NOCICEPTIVE EFFECTS OF MELATONIN IN THE RAT [DOI]
  27. 27 Anti-Inflammatory and Analgesic Effects of an Aqueous Extract ofHarpagophytum procumbens [DOI]
  28. 28 Parthenolide Inhibits Activation of Signal Transducers and Activators of Transcription (STATs) Induced by Cytokines of the IL-6 Family [DOI]
  29. 29 Biological Properties of Flavonoids Pertaining to Inflammation [DOI]
  30. 30 Evaluation of the analgesic, anti‐inflammatory and anti‐diabetic properties of Sclerocarya birrea (A. Rich.) Hochst. stem‐bark aqueous extract in mice and rats [DOI]
  31. 31 Analgesic Activity of the Different Fractions of the Aerial Parts of Commelina benghalensis Linn [DOI]
  32. 32 Studies on the Anti–Inflammatory and Analgesic Properties of Chenopodium Ambrosioides Leaf Extract in Rats [DOI]
  33. 33 Nonsteroidal Antiinflammatory Drugs — Differences and Similarities [DOI]
  34. 34 Improved Anti-Inflammatory Activity of Three New Terpenoids Derived, by Systematic Chemical Modifications, from the Abundant Triterpenes of the Flowery PlantCalendula officinalis [DOI]
  35. 35 Evaluation of analgesic, antipyretic and ulcerogenic effect of Withaferin A
  36. 36 Botanica: The Illustrated A--Z of over 10,000 Garden Plants and How to Cultivate Them [DOI]
  37. 37 Inhibition of the development of collagen-induced arthritis in Wistar rats through vagus nerve suspension: a 3-month observation [DOI]
  38. 38 Trifluoroethoxy‐Coating Improves the Axial Ligand Substitution of Subphthalocyanine [DOI]
  39. 39 Analgesic and Antiinflammatory Effects of Two Chemotypes of Lippia alba: a Comparative Study [DOI]
  40. 40 Anti-inflammatory and analgesic activities of leaf extracts of Landolphia Owariensis [DOI]
  41. 41 Antinociceptive and anti-inflammatory activities ofViburnum opulus [DOI]
  42. 42 Premnazole, an isoxazole alkaloid of Premna integrifolia and Gmelina arborea with anti-inflammatory activity
  43. 43 Antiinflammatory activity of Synurus deltoides [DOI]
  44. 44 Antitumor and antibacterial activity of ethylacetate extract of Ludwigia hyssopifolia linn and its active principle piperine.
  45. 45 Solubility of trifluoroethoxyphthalocyanines and -subphthalocyanines in liquid and supercritical carbon dioxide [DOI]
  46. 46 Synthesis of Benzene-centered Trinuclear Phthalocyanines by Triple-click Chemistry [DOI]
  47. 47 New Therapeutic Approaches for the Treatment of Rheumatoid Arthritis may Rise from the Cholinergic Anti-Inflammatory Pathway and Antinociceptive Pathway [DOI]
  48. 48 Phytochemical screening and antioxidant activity of Leucas aspera.
  49. 49 Essentials of Pharmacology [DOI]
  50. 50 Rheumatoid Arthritis: A New Challenge in Coming Era [DOI]
  51. 51 Biological Properties of Flavonoids Pertaining to Inflammation [DOI]
  52. 52 Naturally occurring antinociceptive substances from plants [DOI]

Cited by 18

Toxicity evaluation, phytochemical characterization and pharmacological effects of aqueous extracts of Combretum collinum leaves: analgesic, antipyretic, and anti-inflammatory activities in animal models

Bunei Kipngetich Kefa, Careen Ihazano Chumba, Daniel Kingori Gathuka · Scientific African · 2025

A review on phytochemical constituents, analytical data, and pharmacological properties of the genus Plumeria

Divyadeepika, Jyoti Joshi · Medicinal Chemistry Research · 2024

Pharmacognostic Standardization and Chromatographic Fingerprint Analysis on Triterpenoids Constituents of the Medicinally Important Plant Plumeria rubra f. rubra by HPTLC technique

Gunja Srivastava, Abhishek Gupta, Manjul Pratap Singh · Pharmacognosy Journal · 2017

Plumeria rubra L.– A review on its ethnopharmacological, morphological, phytochemical, pharmacological and toxicological studies

Tanay Bihani · Journal of Ethnopharmacology · 2021

Showing 4 of 18 known citations — external sources report more than can currently be individually listed.

Related research

Article metrics

Real usage data collected on this platform.

51

Page views

0

PDF downloads

22

Outbound clicks

18

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.