Antineoplastic Activity of the Ethyl Acetate Extracts from Co-cultured Marine and Terrestrial Actinomycetes

Abstract

Treatment of cancer is mainly depending on chemotherapy. A considerable number of antitumor compounds are obtained from microorganisms. Particularly, actinomycetes are the sources of a number of compounds with different biological activities, including antitumor properties. Recent reports by some scientists have revealed a new dimension in producing new bioactive compounds by co-culturing the antibiotic producing actinomycetes. This study was aimed to investigate the effect of co-culturing the antibiotic producing bacteria in liquid broth media among themselves. We used two strains of actinomycetes named ANTS-1 & AIAH-10, which was previously collected from the normal and marine soil of Bangladesh. Antineoplastic activity of co-cultured bacterial metabolites named AIKA-110 was investigated against Ehrlich Ascites Carcinoma (EAC) cells in Swiss Albino mice. AIKA-110 enhanced the mean survival time (MST) of tumor bearing mice at 70.76% and maximum cell growth inhibition was found 74.66% with a dose of 100mg/kg (intraperitoneal). Moreover, hematological parameters of AIKA-110 were found to restore moderately only the high dose. For instance, total RBC count and hemoglobin count was increased but total WBC count was decreased. These findings highlight that the co-culture of microbes triggered to the production of a new compound with promising antineoplastic activities.