Antimicrobial Drug Resistance in Escherichia coli Including an O157:H7 Isolate from Feces of Healthy Goats in Grenada
Victor A. Amadi, Estefania Avendano, Ozioma A. Onyegbule, Zachary Pearl, Stratton Graeme, Ravindra Sharma, Harry Hariharan
Annual Research & Review in Biology · pp. 68–74 · Published 18 Apr 2015
10.9734/ARRB/2015/17129Abstract
Aim: To determine: the occurrence of Escherichia coli including the O157:H7 serotype in feces of healthy goats in 10 randomly selected farms in Grenada and the antimicrobial drug resistance in E. coli isolated from feces of the tested goats. Study Design: During the period of May to July, 2014, fecal samples were obtained from randomly selected healthy goats in 10 farms in Grenada and analyzed in the bacteriology laboratory, in the Pathobiology Department, School of Veterinary Medicine, St. George’s University, Grenada. Methodology: Fecal samples were obtained from 70 randomly selected healthy goats in 10 farms in Grenada and cultured for E. coli and tested for O157:H7 serotype by the presence of non-sorbitol fermenting colonies and a positive reaction to O157-agglutination latex kits. Results: All the 70 tested goats were culture positive for Escherichia coli. A total of 140 E. coli isolates were recovered and analyzed for the presence of non-sorbitol fermenting colonies and O157-agglutination. Of the 140 yielded E. coli, 11 (8%) isolates were non-sorbitol fermenters but only one (<1%) out of the non-sorbitol fermenters gave a positive reaction to the two E. coli O157:H7 latex kits. Antimicrobial susceptibility tests against 12 drugs showed susceptibility of the single E. coli O157:H7 isolate recovered to all the tested antibiotics. Among the non-O157:H7 isolates, the susceptibility rates to amoxicillin-clavulanic acid, ciprofloxacin, enrofloxacin, gentamicin, cefepime and ceftazidime ranged from 99% to 100%. The resistance rate to ampicillin, gentamicin, nalidixic acid, streptomycin, trimethoprim-sulfamethoxazole, and tetracycline observed was very low except to streptomycin (19%). Resistance to two or more antibiotics was observed only in 5% of the 140 E. coli isolates. Conclusion: Our study revealed that presently, healthy goats in Grenada are not major reservoirs for the E. coli O157:H7 serotype and for multiple resistant E. coli strains.
References (29)
- 1 Rapid and Simple Determination of the Escherichia coli Phylogenetic Group [DOI]
- 2 Sex and virulence in Escherichia coli : an evolutionary perspective [DOI]
- 3 Genome sequence of enterohaemorrhagic Escherichia coli O157:H7 [DOI]
- 4 Susceptibility Test Methods: Dilution and Disk Diffusion Methods [DOI]
- 5 A 1-year study of Escherichia coli O157 in cattle, sheep, pigs and poultry [DOI]
- 6 Antimicrobial resistance and resistance gene determinants in clinical Escherichia coli from different animal species in Switzerland [DOI]
- 7 Escherichia coli O157 in cattle and sheep at slaughter, on beef and lamb carcasses and in raw beef and lamb products in South Yorkshire, UK [DOI]
- 8 Detection of Shiga-Like Toxin ( stx 1 and stx 2 ), Intimin ( eaeA ), and Enterohemorrhagic Escherichia coli (EHEC) Hemolysin (EHEC hlyA ) Genes in Animal Feces by Multiplex PCR [DOI]
- 9 Escherichia coli O157:H7 in microbial flora of sheep [DOI]
- 10 Isolation and Characterization of Verocytotoxin-Producing Escherichia coli O157 Strains from Dutch Cattle and Sheep [DOI]
- 11 Characterization of Escherichia coli O157:H7 and other Shiga toxin-producing E. coli serotypes isolated from sheep [DOI]
- 12 Presence of food-borne pathogens on cattle hides [DOI]
- 13 Escherichia coli O157:H7 in faeces from cattle, sheep and pigs in the southwest part of Norway during 1998 and 1999 [DOI]
- 14 Shiga-toxin-producing Escherichia coli in faeces of healthy dairy cows, sheep and goats: prevalence and virulence properties [DOI]
- 15 Verotoxin-Producing Escherichia coli in Spain: Prevalence, Serotypes, and Virulence Genes of O157:H7 and Non-O157 VTEC in Ruminants, Raw Beef Products, and Humans [DOI]
- 16 Occurrence of Escherichia coli O157:H7 in faeces, skin and carcasses from sheep and goats in Ethiopia [DOI]
- 17 Virulence Properties and Serotypes of Shiga Toxin-Producing Escherichia coli from Healthy Australian Slaughter-Age Sheep [DOI]
- 18 Assessing the diversity of the virulence potential ofEscherichia coli isolated from bacteremia in São Paulo, Brazil [DOI]
- 19 Antimicrobial drug resistance of Escherichia coli isolated from poultry abattoir workers at risk and broilers on antimicrobials [DOI]
- 20 Prevalence and molecular characterization of Escherichia coli O157:H7 on Irish lamb carcasses, fleece and in faeces samples [DOI]
- 21 Prevalence of Escherichia coli O157 in lambs at slaughter in Rome, central Italy [DOI]
- 22 Escherichia coli infections [DOI]
- 23 Isolation of a rare Escherichia coli O157:H7 strain from farm animals in Greece [DOI]
- 24 Antimicrobial Resistance and Phylogenetic Groups of Commensal Escherichia Coli Isolates from Healthy Pigs in Grenada
- 25 Mycobacterium avium subspecies paratuberculosis confirmed following serological surveillance of small ruminants in Grenada, West Indies [DOI]
- 26 Verotoxin‐producing Escherichia coli from the faeces of sheep, calves and pigs [DOI]
- 27 Absence of Escherichia coli O157 in a Survey of Wildlife from Trinidad and Tobago [DOI]
- 28 Occurrence of Antibiotic Resistant Escherichia Coli in Green Iguanas (Iguana Iguana ) in Grenada, West Indies
- 29 Antimicrobial Susceptibility Profiles of Escherichia coli Recovered from Feces of Young Healthy Domestic Pigs in Grenada, West Indies [DOI]
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