Antimicrobial Activity of Antibiotics, Oregano and Thyme Essential Oils against Extended Spectrum Beta Lactamase Producing Klebsiella pneumoniae Clinical Isolates
Snježana Hodžić, Amela Hercegovac, Nijaz Tihić, Darja Husejnagić, Aldijana Avdić, Merima Ibišević, Melisa Kadrić
Journal of Advances in Microbiology · pp. 112–119 · Published 16 Dec 2021
10.9734/jamb/2021/v21i1230419Abstract
Aims: The aims of this study was to investigate the susceptibility of extended-spectrum beta-lactamase (ESBL) producing Klebsiella pneumoniae clinical isolates to antibiotics and essential oils - Origanum compactum, Origanum majorana and Thymus serpyllum. Study Design: Study included 30 isolates of Klebsiella pneumoniae obtained from clinical material provided from the University Clinical Center Tuzla. Place and Duration of Study: Department of Biology, Faculty of Science, University of Tuzla, BiH, between September 2019 to September 2020. Methodology: Antibiotic susceptibility testing was performed by the Kirby-Bauer disk diffusion method. The following commercially available antibiotic discs were used: amoxicillin (30µg), cefalexin (30 µg), gentamicin (10 µg), amikacin (30 µg), imipenem (10 µg), piperacillin (75µg), ampicilin (10 µg), meropenem (10 µg), ciprofloksacin (10 µg), ceftazidim (30 µg), cefotaksim (30 µg), ceftriaxone (30 µg), cefepime (30 µg) and aztreonam (30 µg). The antibacterial effect of the essential oils was tested for ESBL K. pneumoniae isolates using the diffusion method according to Clinical laboratory standards institute (CLSI) guidelines. Results: O. compactum and O. majorana essential oils showed the same antimicrobial activity with 80.0% effect on ESBL K. pneumoniae isolates, Thymus serpyllum EO showed antimicrobial activity of 60.0%. The lowest MIC value had the O. compactum essential oil (MIC 6 mg/ml-10.5 mg/ml), followed by the T. serpyllum (MIC 17.2 mg/ml-43 mg/ml), while the O. majorana essential oil showed MIC values in range from 11 mg/ml to 39 mg/ml. Conclusion: The results of the study showed the exceptional sensitivity of ESBL K. pneumoniae clinical isolates to the essential oils from Origanum and Thymus genera, which highly suggests their potential application in the struggle against these pathogens in the future.
Cited by 0
No indexed citations yet.
Related research
- Genotypic Analysis of Klebsiella pneumoniae among Patients Admitted in Critical Care Settings in a Tertiary Care Center of Bangladesh — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
- Molecular Detection of the Arr-2 Gene in Escherichia coli and Klebsiella pneumoniae Resistant to Rifampicin in Abidjan, Côte D'Ivoire — shares topic coverage
- Detection of Hypervirulent Klebsiella pneumoniae in Tanta University Hospital, Egypt — shares topic coverage
- Phenotypic Study on Some Virulence Factors and Molecular Screening of Aminoglycoside Resistance among Klebsiella pneumoniae Strains Isolated from Urinary Tract Infections in Pediatric Cases in Egypt — shares topic coverage
Article metrics
Real usage data collected on this platform.
10
Page views
0
PDF downloads
0
Outbound clicks
0
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.