Anticariogenic Activity of Copper Nanoparticles Synthesized Using Blue Tea

Abstract

Background: Nanotechnology is rapidly growing in various fields of science like medicinal, agricultural and physical and material sciences. Copper nanoparticles are particularly attractive because of copper’s high natural abundance and low cost and the practical and straightforward multiple ways of preparing copper based nanomaterials. Aim: To assess the anticariogenic activity of copper nanoparticles synthesized using blue tea. Materials and Methods: The blue tea powder was acquired. An aqueous extract was prepared and mixed with copper sulphate for copper nanoparticles formation and centrifuged for 10 minutes. The extract was then placed in the well cultured agar plates against C. albicans, S. mutans, S. aureus and E. faecalis and incubated for 24 hours. The zones of inhibition were then calculated. Results: Against S. aureus, 25μl showed 10 mm of zone of inhibition, 50μl showed 10 mm of zone of inhibition and 100μl showed 12 mm of zone of inhibition. 21 mm of zone of inhibition were noted against the antibiotic. Against C. albicans, 25 μl showed 25 mm of zone of inhibition, 50 μl showed 26 mm of zone of inhibition and 100 μl showed 30 mm of zone of inhibition. 12 mm of zone of inhibition were noted against the antibiotic. Against S. mutans, 25 μl showed 10 mm of zone of inhibition, 50 μl showed 10 mm of zone of inhibition and 100 μl showed 25 mm of zone of inhibition. 21 mm of zone of inhibition were noted against the antibiotic. Against E. faecalis, 25 μl showed 10 mm of zone of inhibition, 50 μl showed 13 mm of zone of inhibition and 100 μl showed 14 mm of zone of inhibition. 37 mm of zone of inhibition were noted against the antibiotic. Conclusion: The blue tea mediated copper nanoparticles showed anticariogenic activity against S. mutans, C. albicans, E. faecalis and S. aureus and therefore can be used for clinical application.