Anticancer Effect of Moringa oleifera Leaf Extract on A2780 Ovarian Cancer Cell Line

Abstract

In this study we investigated anticancer effect of Moringa oleifera leaf extract on A2780 ovarian cancer cell line. The leave of Moringa oleifera were grinded into powder and the phytochemicals were extracted using 80% ethanol. For fractionation and partitioning process four kinds of solvents were used in order: Petroleum ether, Dichloromethane, Ethyl acetate and Water extract until the extract is colorless or lightened. For cell proliferation assay all samples were dissolved in DimethylSulfoxide. Anticancer effect of Moringa oleifera were tested on A2780 ovarian cell line with four different extracts using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide. The final result showed that on Petroleum Ether, Dichloromethane and Ethyl Acetate extracts as the concentration increases from 1 µg/ml, 2 µg/ml, 3 µg/ml, 4 µg/ml inhibited A2780, despite that in water extracts when the concentration decreases the inhibitory effect increases, however, Dichloromethane Extract has higher anticancer effect on A2780 ovarian cancer cell line with insignificant cytotoxicity to the normal cell.