Antibiotic Susceptibility Testing, Plasmid Detection and Curing of Clinically Isolated Enterococcus Species
G. A. C. Ezeah, M. C. Ugwu, A. O. Ekundayo, O. F. Odo, O. C. Ike, R. A. Akpe
Journal of Advances in Microbiology · pp. 1–20 · Published 10 Jun 2019
10.9734/jamb/2019/v16i330125Abstract
Vancomycin resistant enterococci (VRE) are a major medical concern globally. Their significantly greater prevalence and the ability to transfer resistance to vancomycin from other bacteria made them an object of interest and intense research. The isolates of Enterococcus sp. were subjected to antibiotic susceptibility testing before curing. The three Enterococcus species exhibited different antibiotic resistance profile. Pre-curing antibiotic resistance of nosocomial isolates compared with community acquired isolates revealed that high percentage of the nosocomial isolates were resistant to antibiotics compared to community isolate. Post-curing antibiograms of the isolates showed different resistant and susceptibility pattern. Also, DNA plasmid pre-curing and post curing analysis of the isolates showed different resistance pattern. Six of the 15 representative isolates selected on the basis of their high pre-curing antibiotic resistance for plasmid analysis with 0.8% agarose electrophoresis were positive for plasmid DNA. Four (4) of the positive isolates (E. faecium, E. faecium, E. faecalis, and E. avium) had plasmid fragment of greater than 1000 bp while two (2) of them (E. faecalis and E. faecalis) had fragments of between 100 and 500 bp. The remaining nine (9) had no plasmid DNA. The study revealed the pathogenicity factors demonstrated with the enterococcal isolates.
References (12)
- 1 Bacterial Biofilms: A Common Cause of Persistent Infections [DOI]
- 2 Quorum Sensing in Bacteria [DOI]
- 3 The ecology, epidemiology and virulence of Enterococcus [DOI]
- 4 Health Care Costs and Mortality Associated with Nosocomial Diarrhea Due to Clostridium difficile [DOI]
- 5 Cystic fibrosis pathogenesis and the role of biofilms in persistent infection [DOI]
- 6 Ace Is a Collagen-binding MSCRAMM from Enterococcus faecalis [DOI]
- 7 Molecular Epidemiology of the fsr Locus and of Gelatinase Production among Different Subsets of Enterococcus faecalis Isolates [DOI]
- 8 In Vitro Activity of 19 Antimicrobial Agents against Enterococci from Healthy Subjects and Hospitalized Patients and Use of an ace Gene Probe from Enterococcus faecalis for Species Identification [DOI]
- 9 Fsr-Independent Production of Protease(s) May Explain the Lack of Attenuation of an Enterococcus faecalis fsr Mutant Versus a gelE - sprE Mutant in Induction of Endocarditis [DOI]
- 10 Pattern of antibiotic usage by adult populations in the city of Benin, Nigeria [DOI]
- 11 Nosocomial infections: urinary tract infection in patients with indwelling urinary catheter [DOI]
- 12 INCIDENCE OF MULTIPLE ANTIBIOTIC RESISTANCE AND PLASMID CARRIAGE AMONG ENTEROCOCCUS FAECALIS ISOLATED FROM THE HANDS OF HEALTH CARE WORKERS IN SELECTED HOSPITALS IN EKITI, ONDO AND OSUN STATES, NIGERIA
Cited by 8
Oluwatosin Qawiyy Orababa, Jeffry Difiye Soriwei, Samuel Oluwamayowa Akinsuyi · Porto Biomedical Journal · 2021
Kome Otokunefor, Blessing O. Famakin, Dorothy O. Douglas · Bulletin of the National Research Centre · 2020
I. Kumari, S. K. Tiwari · Applied Biochemistry and Microbiology · 2025
Showing 3 of 8 known citations — external sources report more than can currently be individually listed.
Related research
- Antibiotic Susceptibility Pattern of Group A Beta-Haemolytic Streptococcal Throat Isolates of Primary School Children in Rivers State, Nigeria — shares topic coverage
- Prevalence, Aetiological Agents and Antimicrobial Resistance Patterns of Urinary Tract Infections among Pregnant Women Attending Mother and Child Hospital, Akure, South-West Nigeria — shares topic coverage
- Incidence of Enterobacteriaceae in Retail Meat Samples and Their Antibiotic Susceptibility — shares topic coverage
- Bacterial Aetiology and Antibiotic Susceptibility Profile of Post-Operative Sepsis among Surgical Patients in a Tertiary Hospital in Rural Eastern Uganda — shares topic coverage
- Traditional and Molecular Identification of Bacterial Contamination from Honey (Processed and Unprocessed) and Their Control by Phytotherapy — shares topic coverage
Article metrics
Real usage data collected on this platform.
13
Page views
0
PDF downloads
1
Outbound clicks
8
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.