Anti-Inflammatory Effects of New Flavonoids from Streptomyces sp. BT01 in Lipopolysaccharide-Stimulated RAW 264.7 Murine Macrophages via Inhibition of NF-KappaB Activation
Thongchai Taechowisan, Srisakul Chanaphat, Wanwikar Ruensamran, Waya S. Phutdhawong
Current Journal of Applied Science and Technology · pp. 4201–4222 · Published 7 Aug 2014
10.9734/BJAST/2014/12049Abstract
Most flavonoids are known to have anti-oxidant, anti-bacterial and analgesic properties. In this study, the new flavonoids, 7-methoxy-3,3’,4’,6-tetrahydroxyflavone (1) and 2’,7-dihydroxy-4’5’-dimethoxyisoflavone (2) isolated from Streptomyces sp. BT01 inhibited the pro-inflammatory mediators including cytokines by blocking nuclear factor-kappaB (NF-κB) signalling in lipopolysaccharide (LPS)-stimulated RAW 264.7 cells. These flavonoids suppressed mRNA and protein expression of inducible nitric oxide synthase (iNOS) and cyclooxygenase-2 (COX-2) in LPS-stimulated RAW 264.7 cells. The molecular mechanism was associated with the inhibition of NF-κB activation. These results suggest that these flavonoids have anti-inflammatory effects by suppressing expression of iNOS, COX-2 and cytokines by blocking the NF-κB signalling in LPS-stimulated RAW 264.7 cells.
References (41)
- 1 Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays [DOI]
- 2 Analysis of nitrate, nitrite, and [15N]nitrate in biological fluids [DOI]
- 3 Antimicrobial activity of flavonoids [DOI]
- 4 NITRIC OXIDE AND MACROPHAGE FUNCTION [DOI]
- 5 Elevated Circulating Levels of Tumor Necrosis Factor in Severe Chronic Heart Failure [DOI]
- 6 LPS induction of gene expression in human monocytes [DOI]
- 7 Prostaglandin synthase 2 [DOI]
- 8 Colorectal cancer prevention and treatment by inhibition of cyclooxygenase-2 [DOI]
- 9 Flavonoids as anti-inflammatory agents: implications in cancer and cardiovascular disease [DOI]
- 10 Anti-inflammatory Plant Flavonoids and Cellular Action Mechanisms [DOI]
- 11 Possible new role for NF-κB in the resolution of inflammation [DOI]
- 12 Nuclear Factor κB Is a Molecular Target for Sulforaphane-mediated Anti-inflammatory Mechanisms [DOI]
- 13 Modulation of acute inflammation by endogenous nitric oxide [DOI]
- 14 Lipopolysaccharide (LPS)-induced Macrophage Activation and Signal Transduction in the Absence of Src-Family Kinases Hck, Fgr, and Lyn [DOI]
- 15 Chemopreventive properties of trans-resveratrol are associated with inhibition of activation of the IkappaB kinase.
- 16 NF- k appa B regulates IL-1 beta transcription through a consensus NF- k appa B binding site and a nonconsensus CRE-like site. [DOI]
- 17 Comparative studies on the suppression of nitric oxide synthase by curcumin and its hydrogenated metabolites through down-regulation of IκB kinase and NFκB activation in macrophages [DOI]
- 18 Inhibition of nuclear factor kappaB activation in PC3 cells by genistein is mediated via Akt signaling pathway.
- 19 Inhibition of LPS-Induced iNOS, COX-2 and Cytokines Expression by Poncirin through the NF-.KAPPA.B Inactivation in RAW 264.7 Macrophage Cells [DOI]
- 20 Chlorogenic acid inhibits lipopolysaccharide-induced cyclooxygenase-2 expression in RAW264.7 cells through suppressing NF-κB and JNK/AP-1 activation [DOI]
- 21 Circulating interleukin‐6 mediates the febrile response to localised inflammation in rats [DOI]
- 22 Isolation of endophytic actinomycetes from selected plants and their antifungal activity [DOI]
- 23 Functional Components in Soybean Cake and Their Effects on Antioxidant Activity [DOI]
- 24 M ast C ells and O ral I nflammation [DOI]
- 25 Anti-inflammatory activity of 4-methoxyhonokiol is a function of the inhibition of iNOS and COX-2 expression in RAW 264.7 macrophages via NF-κB, JNK and p38 MAPK inactivation [DOI]
- 26 Genistein Inhibits NF-kB Activation in Prostate Cancer Cells [DOI]
- 27 Nitric oxide and inflammatory joint diseases [DOI]
- 28 Interleukin‐6 production by human gingival fibroblasts [DOI]
- 29 Effects of propolis from Brazil and Bulgaria on fungicidal activity of macrophages against Paracoccidioides brasiliensis [DOI]
- 30 Suppression of Constitutive and Tumor Necrosis Factor α-Induced Nuclear Factor (NF)-κB Activation and Induction of Apoptosis by Apigenin in Human Prostate Carcinoma PC-3 Cells: Correlation with Down-Regulation of NF-κB-Responsive Genes [DOI]
- 31 IL-1 and TNF-α Play a Pivotal Role in the Host Immune Response in a Mouse Model ofStaphylococcus aureus-Induced Experimental Brain Abscess [DOI]
- 32 Bacterial endotoxins and pathogenesis of Gram-negative infections: current status and future direction [DOI]
- 33 Flower extract of Panax notoginseng attenuates lipopolysaccharide-induced inflammatory response via blocking of NF-κB signaling pathway in murine macrophages [DOI]
- 34 Inactivation of Akt and NF-κB Play Important Roles During Indole-3-Carbinol-Induced Apoptosis in Breast Cancer Cells [DOI]
- 35 Antibacterial activity of new flavonoids from Streptomyces sp. BT01; an endophyte in Boesenbergia rotunda (L.) Mansf. [DOI]
- 36 Anti-inflammatory activity of lansais from endophyticStreptomycessp. SUC1 in LPS-induced RAW 264.7 cells [DOI]
- 37 Phytoestrogens in cancer prevention and therapy--mechanisms of their biological activity.
- 38 Treatment of mice with IL-1 before infection increases resistance to a lethal challenge with Salmonella typhimurium. The effect correlates with the resistance allele at the Ity locus. [DOI]
- 39 Inhibition of lipopolysaccharide-induced inducible nitric oxide synthase expression by a novel compound, mercaptopyrazine, through suppression of nuclear factor-kappaB binding to DNA [DOI]
- 40 Anti-inflammatory effects of lansai C and D cause inhibition of STAT-1 and NF-κB activations in LPS-induced RAW 264.7 cells [DOI]
- 41 Eicosanoids [DOI]
Cited by 5
Ankika Bhakat, Priyanka Das, Mitrabrata Goswami · Sustainable Landscape Planning and Natural Resources Management · 2025
Lawan Siangjong, Paranee Meetam · Pharmacia · 2024
Showing 2 of 5 known citations — external sources report more than can currently be individually listed.
Related research
- Comparative Phytochemical Profiling and Antimicrobial Efficacy of Ganoderma and Shiitake Mushrooms: A Microbiological Perspective — shares topic coverage
- A Comparative Study on Potential Antioxidants and Antioxidant Activity in Raw and Cooked Selected Locally Grown Legumes in Sri Lanka — shares topic coverage
- Effect of Fortification Breadsticks with Milk Thistle Seeds Powder on Chemical and Nutritional Properties — shares topic coverage
- Production and Quality Evaluation of Selected Spices [Ginger (Zingiber officinale), Garlic (Allium Sativum), Tumeric (Curcuma longa) and Clove (Syzygium aromaticum)] — shares topic coverage
- Evaluation of the Antioxidant Properties of Some Commonly Eaten Vegetables in Akwa Ibom State of Nigeria — shares topic coverage
Article metrics
Real usage data collected on this platform.
10
Page views
0
PDF downloads
0
Outbound clicks
5
Citations
Views over time
Views by country
Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".
Traffic sources
Referring site, by host.
Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.