Skip to content
Research Article Open access CC BY 4.0

Andrographis Echioides Molecular Characterization and Phylogenetic Study Using DNA Barcoding Technique

Prashant Hande, B. S. Ajit Kumar, Vikas Jha, Anjali Bhosale, Subodh Gangurde

European Journal of Medicinal Plants · pp. 48–56 · Published 10 Aug 2022

10.9734/ejmp/2022/v33i830484

Abstract

With the advancement in various molecular diagnostic tools, DNA Barcoding has emerged as a gold standard molecular diagnostic tool across the globe. Since ancient times, medicinal plants have been widely used in Indian Ayurvedic medicine for treating a variety of ailments. Plants of the genus Andrographis have been extensively used for treating different types of ailments. In this study, rarely studied medicinal plant species were isolated, sequenced at the genetic level and studied for their evolutionary characteristics using phylogenetic analysis. In the present study, the identity of A. echioides was confirmed by targeting different barcoding genes such as ribulose-bisphosphate carboxylase, internal transcribed spacer, RNA polymerase-beta subunit, maturase K, and photosystem II protein D1 genes using a phylogenetic approach. After successful isolation and amplification of genomic DNA, specific primers were utilised for sequencing of each barcoding gene, followed by nucleotide BLAST analysis to determine the sequence percent identity of each gene with that from other plant species. The best homologs were then utilised for conducting phylogenetic analysis which confirmed the identity of the plant as Andrographis echioides.

Andrographis echioides DNA barcoding phylogeny

References (17)

  1. 1 The neighbor-joining method: a new method for reconstructing phylogenetic trees. [DOI]
  2. 2 A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences [DOI]
  3. 3 Barcoding animal life: cytochrome c oxidase subunit 1 divergences among closely related species [DOI]
  4. 4 The tortoise and the hare II: relative utility of 21 noncoding chloroplast DNA sequences for phylogenetic analysis [DOI]
  5. 5 Ribosomal ITS sequences and plant phylogenetic inference [DOI]
  6. 6 A Two-Locus Global DNA Barcode for Land Plants: The Coding rbcL Gene Complements the Non-Coding trnH-psbA Spacer Region [DOI]
  7. 7 Multiple Multilocus DNA Barcodes from the Plastid Genome Discriminate Plant Species Equally Well [DOI]
  8. 8 Identifying spiders through DNA barcodes [DOI]
  9. 9 Land plants and DNA barcodes: short-term and long-term goals [DOI]
  10. 10 Progress towards DNA barcoding of fungi [DOI]
  11. 11 Use of nuclear genes for phylogeny reconstruction in plants [DOI]
  12. 12 Selecting barcoding loci for plants: evaluation of seven candidate loci with species‐level sampling in three divergent groups of land plants [DOI]
  13. 13 The use of chloroplast DNA to resolve plant phylogenies: noncoding versus rbcL sequences. [DOI]
  14. 14 A DNA mini‐barcode for land plants [DOI]
  15. 15 Chemical Constituents from Andrographis echioides and Their Anti-Inflammatory Activity [DOI]
  16. 16 Morphological and molecular description of parasitic leeches (Annelida: Hirudinea) isolated from rice field of Bandar Anzali, North of Iran [DOI]
  17. 17 ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF Andrographis echioides (L.) Nees LEAVES EXTRACTS [DOI]

Cited by 0

No indexed citations yet.

Related research

Article metrics

Real usage data collected on this platform.

54

Page views

0

PDF downloads

22

Outbound clicks

0

Citations

Views over time

Views by country

Approximate, from request IP at view time — not citizenship or institution. Countries with fewer than 5 views are grouped as "Other".

Traffic sources

Referring site, by host.

Views and downloads exclude known bots/crawlers. Citations combines this platform's own DOI-resolved index with each external source's own reported total — see Cited by above for individually listed citing works. Last refreshed 0 seconds ago.