An Overview of Cultural, Molecular and Metagenomic Techniques in Description of Microbial Diversity

References (59)

1. 1 Profiling of complex microbial populations by denaturing gradient gel electrophoresis analysis of polymerase chain reaction-amplified genes coding for 16S rRNA [DOI]
2. 2 Phylogenetic identification and in situ detection of individual microbial cells without cultivation [DOI]
3. 3 Functional interactions between the gut microbiota and host metabolism [DOI]
4. 4 Environmental Genome Shotgun Sequencing of the Sargasso Sea [DOI]
5. 5 Microbial diversity in the deep sea and the underexplored “rare biosphere” [DOI]
6. 6 Metagenomics: Application of Genomics to Uncultured Microorganisms [DOI]
7. 7 Classification and Characterization of Heterotrophic Microbial Communities on the Basis of Patterns of Community-Level Sole-Carbon-Source Utilization [DOI]
8. 8 Virioplankton: Viruses in Aquatic Ecosystems [DOI]
9. 9 The Sorcerer II Global Ocean Sampling Expedition: Northwest Atlantic through Eastern Tropical Pacific [DOI]
10. 10 Molecular biological access to the chemistry of unknown soil microbes: a new frontier for natural products [DOI]
11. 11 Direct detection of DNA methylation during single-molecule, real-time sequencing [DOI]
12. 12 The species concept for prokaryotes [DOI]
13. 13 Metagenomics - a guide from sampling to data analysis [DOI]
14. 14 Automated Approach for Ribosomal Intergenic Spacer Analysis of Microbial Diversity and Its Application to Freshwater Bacterial Communities [DOI]
15. 15 A Primer on Metagenomics [DOI]
16. 16 A New Approach To Utilize PCR–Single-Strand-Conformation Polymorphism for 16S rRNA Gene-Based Microbial Community Analysis [DOI]
17. 17 High‐throughput DNA sequencing – concepts and limitations [DOI]
18. 18 PCR-Induced Sequence Artifacts and Bias: Insights from Comparison of Two 16S rRNA Clone Libraries Constructed from the Same Sample [DOI]
19. 19 Analysis of microbial community functional diversity using sole-carbon-source utilisation profiles – a critique [DOI]
20. 20 The guanine and cytosine content of genomic DNA and bacterial evolution. [DOI]
21. 21 Evaluation of Methods for the Extraction and Purification of DNA from the Human Microbiome [DOI]
22. 22 Opening the black box of soil microbial diversity [DOI]
23. 23 PCR Biases Distort Bacterial and Archaeal Community Structure in Pyrosequencing Datasets [DOI]
24. 24 A Culture-Independent Sequence-Based Metagenomics Approach to the Investigation of an Outbreak of Shiga-Toxigenic Escherichia coli O104:H4 [DOI]
25. 25 Raman‐FISH: combining stable‐isotope Raman spectroscopy and fluorescence in situ hybridization for the single cell analysis of identity and function [DOI]
26. 26 Molecular Techniques for Determining Microbial Diversity and Community Structure in Natural Environments [DOI]
27. 27 Improved group-specific PCR primers for denaturing gradient gel electrophoresis analysis of the genetic diversity of complex microbial communities [DOI]
28. 28 Unlocking the potential of metagenomics through replicated experimental design [DOI]
29. 29 Polymerase chain reaction primers miss half of rRNA microbial diversity [DOI]
30. 30 Marine microbial diversity and ecology: importance and future perspectives
31. 31 Monitoring of microbial hydrocarbon remediation in the soil [DOI]
32. 32 Metagenomic Analyses of Drinking Water Receiving Different Disinfection Treatments [DOI]
33. 33 Effects of polymerase, template dilution and cycle number on PCR based 16 S rRNA diversity analysis using the deep sequencing method [DOI]
34. 34 Analytical Tools and Databases for Metagenomics in the Next-Generation Sequencing Era [DOI]
35. 35 Stable isotope probing in the metagenomics era: A bridge towards improved bioremediation [DOI]
36. 36 Metagenomic comparison of direct and indirect soil DNA extraction approaches [DOI]
37. 37 Diagnostic metagenomics: potential applications to bacterial, viral and parasitic infections [DOI]
38. 38 Next-generation sequencing technologies: breaking the sound barrier of human genetics [DOI]
39. 39 POLYMERASE CHAIN REACTION: METHODS, PRINCIPLES AND APPLICATION [DOI]
40. 40 Methods for Analyzing Diversity of Microbial Communities in Natural Environments [DOI]
41. 41 Modeling ecological drivers in marine viral communities using comparative metagenomics and network analyses [DOI]
42. 42 From next-generation sequencing to systematic modeling of the gut microbiome [DOI]
43. 43 Cultivating the uncultivated: a community genomics perspective [DOI]
44. 44 Identification of Carbohydrate Metabolism Genes in the Metagenome of a Marine Biofilm Community Shown to Be Dominated by Gammaproteobacteria and Bacteroidetes [DOI]
45. 45 Marine metagenomics, a valuable tool for enzymes and bioactive compounds discovery [DOI]
46. 46 Pyrosequencing-An Alternative to Traditional Sanger Sequencing [DOI]
47. 47 Modern molecular approaches for analyzing microbial diversity from mushroom compost ecosystem [DOI]
48. 48 Databases of the marine metagenomics [DOI]
49. 49 Fatty acid methyl ester (FAME) profiles as a tool to investigate community structure of two agricultural soils [DOI]
50. 50 The use of 16s rDNA methods in soil microbial ecology [DOI]
51. 51 Molecular Techniques to Assess Microbial Community Structure, Function, and Dynamics in the Environment [DOI]
52. 52 EBI metagenomics in 2016 - an expanding and evolving resource for the analysis and archiving of metagenomic data [DOI]
53. 53 Evaluation of prokaryotic diversity by restrictase digestion of 16S rDNA directly amplified from hypersaline environments [DOI]
54. 54 Nutritive Value of Common Wild Edible Mushrooms from Southern Nigeria
55. 55 Metagenomics and its application in soil microbial community studies: biotechnological prospects.
56. 56 Nutrient Profile and Phytochemical Analysis of Commercially Cultivated Oyster Mushroom in Calabar, South-South Nigeria [DOI]
57. 57 Microbial Diversity of a Remote Aviation Fuel Contaminated Sediment of a Lentic Ecosystem in Ibeno, Nigeria [DOI]
58. 58 MICROBIAL ECOLOGY OF COMPOST ECOSYSTEM: WITH SPECIAL REFERENCE TO MUSHROOM COMPOST [DOI]
59. 59 Metagenomic analysis of bacterial community composition among the cave sediments of Indo-Burman biodiversity hotspot region [DOI]

Cited by 57

Showing 22 of 57 known citations — external sources report more than can currently be individually listed.

Related research

• Comparison of Molecular Methods of Microbial Serotyping — shares topic coverage
• Bacterial Succession Studies during Fermentation of African Locust Bean (Parkia biglobosa) to Iru Using Molecular Methods — shares topic coverage
• Prevalence of Malaria and Plasmodium Species Composition in the Western Highlands of the North West Region, Cameroon — shares topic coverage
• An Improved Protocol for Extraction of Metagenomic DNA from High Humus, Alkaline and Saline Soil of Chinampa for T-RFLP Fingerprinting Analysis — shares topic coverage
• Comparative Bacterial Metagenomics of Cnidoscolus aconitifolius (Mill.) I. M. Johnston and Other Leafy Vegetables — shares topic coverage