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Research Article Open access CC BY 4.0

Ameliorative Potentials of Persea americana Mill (Avocado) Seed Extract on Acetaminophen-induced Hepatic Insufficiency in Rats

Enyinnaya A. Ogbonnaya, Donatus C. Belonwu, Ogechukwu E. Ezim, Uzoma Umeh

Asian Journal of Research in Biochemistry · pp. 28–39 · Published 20 Mar 2024

10.9734/ajrb/2024/v14i2281

Abstract

Aim: The ability of ethylacetate extract of Persea americana Mill (avocado) seed to ameliorate the hepatotoxic effect of acetaminophen in rats was assessed. Methodology: Thirty experimental rats were placed in 6 groups of 5 animals per group. Two animal groups received distilled water and the drug vehicle, dimethyl sulphoxide (DMSO). Four other groups received acetaminophen (750 mg/kg bw/day) in DMSO for 3 days with 3 of the 4 groups receiving, after 3 days of acetaminophen administration and for 11 days, Persea americana seed extracts (150-, 350 mg/kg bw) and silymarin (200 mg/kg bw). Animals were sacrificed and both serum and liver tissue analysed for markers of liver integrity (AST, ALT, ALP), liver function (total protein, albumin, total cholesterol, triglyceride), antioxidant status (SOD, Catalase) and liver architecture. Results: The result showed induction of hepatotoxicity by acetaminophen with increased AST, ALT, ALP and decreased SOD and CAT activities. Hepatotoxicity was also observed as increase in total and conjugated bilirubin and decrease in total cholesterol and triglyceride. Administration of Persea americana seed ethylacetate extract reversed the status of the parameters towards normal (except for AST, total protein and albumin). Also the liver architecture which hitherto revealed inflammation, fatty changes and hepatocyte necrosis also reversed to mild inflammation to normal histology. The action of the extract was dose dependent and its effectiveness comparable to silymarin (200 mg/kg bw). Conclusion: The ethylacetate extract of Persia americana showed ameliorative effect against acetaminophen-induced hepatotoxicity at higher dose, with potentials to reverse disruptions in liver integrity and function, and restore balance in endogenous antioxidant system.

Persea americana mill acetaminophen hepatotoxicity antioxidant silymarin

References (37)

  1. 1 The Role of Superoxide Anion in the Autoxidation of Epinephrine and a Simple Assay for Superoxide Dismutase [DOI]
  2. 2 Ethanolic Extract of Propolis (EEP) Enhances the Apoptosis- Inducing Potential of TRAIL in Cancer Cells [DOI]
  3. 3 Silymarin as Supportive Treatment in Liver Diseases: A Narrative Review [DOI]
  4. 4 Liver injury induced by paracetamol and challenges associated with intentional and unintentional use [DOI]
  5. 5 Silymarin prevents acetaminophen-induced hepatotoxicity in mice [DOI]
  6. 6 Measurement and clinical usefulness of bilirubin in liver disease [DOI]
  7. 7 Role of silymarin as antioxidant in clinical management of chronic liver diseases: a narrative review [DOI]
  8. 8 Avocado seed discoveries: Chemical composition, biological properties, and industrial food applications [DOI]
  9. 9 Schisandra fruits for the management of drug-induced liver injury in China: A review [DOI]
  10. 10 Effects of nerol on paracetamol-induced liver damage in Wistar albino rats [DOI]
  11. 11 Silymarin: not just another antioxidant [DOI]
  12. 12 Phytochemicals properties of avocado seed: A review [DOI]
  13. 13 Silymarin is an ally against insulin resistance: A review [DOI]
  14. 14 Antioxidative and Anti-Inflammatory Protective Effects of Fucoxanthin against Paracetamol-Induced Hepatotoxicity in Rats [DOI]
  15. 15 Comparative role of acetaminophen, carbon tetrachloride and thioacetamide in development of fibrosis in rats [DOI]
  16. 16 Acute Toxicity and Genotoxic Activity of Avocado Seed Extract (Persea americana Mill., c.v. Hass) [DOI]
  17. 17 Evaluation of Hepatoprotective Activity of Adansonia digitata Extract on Acetaminophen‐Induced Hepatotoxicity in Rats [DOI]
  18. 18 l-Methionine and silymarin: A comparison of prophylactic protective capabilities in acetaminophen-induced injuries of the liver, kidney and cerebral cortex [DOI]
  19. 19 Cinnamon oil against acetaminophen-induced acute liver toxicity by attenuating inflammation, oxidative stress and apoptosis [DOI]
  20. 20 Hepatoprotective effect of Pandanus odoratissimus seed extracts on paracetamol-induced rats [DOI]
  21. 21 Acetaminophen-induced liver injury: Implications for temporal homeostasis of lipid metabolism and eicosanoid signaling pathway [DOI]
  22. 22 Subacute Oral Toxicity Assesment of Ethanol Extract ofMariposa christia vespertilionisLeaves in Male Sprague Dawley Rats [DOI]
  23. 23 Silymarin Protects against Acute Liver Injury Induced by Acetaminophen by Downregulating the Expression and Activity of the CYP2E1 Enzyme [DOI]
  24. 24 The potential protective role of folic acid against acetaminophen-induced hepatotoxicity and nephrotoxicity in rats [DOI]
  25. 25 A dual role of inflammation in acetaminophen-induced liver injury [DOI]
  26. 26 Evaluating the hepatoprotective, Ameliorative and Antioxidant Potentials of the Crude Aqueous Leafy Extracts of Mangifera Indica Plant Against Acute paracetamol-induced Hepatotoxicity in a Mouse Model [DOI]
  27. 27 Silymarin prevents acetaminophen-induced hepatotoxicity via up-regulation of the glutathione conjugation capacity in mice [DOI]
  28. 28 A 13-week subchronic toxicity study of acetaminophen using an obese rat model [DOI]
  29. 29 [Retracted] Preventive Effects of Mandarin Fruit Peel Hydroethanolic Extract, Hesperidin, and Quercetin on Acetaminophen‐Induced Hepatonephrotoxicity in Wistar Rats [DOI]
  30. 30 Bioactive Composition and Acute Oral Toxicity Studies on Persea Americana Seed Ethyl Acetate Fraction [DOI]
  31. 31 Reversal of acetaminophen-generated oxidative stress and concomitant hepatotoxicity by a phytopharmaceutical product [DOI]
  32. 32 In Vitro Antioxidant Activities and the Therapeutic Potential of Some Newly Synthesized Chalcones Against 4-Acetaminophenol Induced Hepatotoxicity in Rats [DOI]
  33. 33 Ameliorative effect of ethanolic extract of Limnophila rugosa (Scrophulariaceae) in paracetamol- and carbon tetrachloride-induced hepatotoxicity in rats [DOI]
  34. 34 Mechanism of protection of rat hepatocytes from acetaminophen-induced cellular damage by ethanol extract of Aerva lanata [DOI]
  35. 35 The ameliorative effect of the solvent extracts of Ocimum basilicum against acetaminophen-induced liver damage in albino rats [DOI]
  36. 36 Atherogenic and Haematologic Indices of Paracetamol- Overdosed Albino Rats Treated With Aqueous Leaf Extracts of Euphorbia Heterophylla and Jatropha Curcas [DOI]
  37. 37 "Insights From a Rat Model of Paracetamol-Induced Hepatotoxicity into the Molecular Mechanisms of Silymarin and Resveratrol Combination Therapy for Protecting Liver Function" [DOI]

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Mohammed A Abdel-Rasol, Nadia M El-Beih, Wael M El-Sayed · Mediterranean Journal of Nutrition and Metabolism · 2025

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