Alleviated Immunological Activities of Wistar Rat Peritoneal Neutrophils and Macrophages by Polysaccharide Rich Extract from the Fruit Bodies of Microporus vernicipes (Polyporales)

Abstract

Aim: This study aimed to assess the effect of polysaccharides from fruit body of Microporous vernicipes (Polyporales) on immunological activities of macrophages and neutrophils. Methodology: Polysaccharides were extracted using hot water (PHW) and cool water (PCW) and tested on the activities of peritoneal macrophage and neutrophil in response of lipopolysaccharides. The effect of the polysaccharides was examined In vitro to assess the phagocytic activities by evaluating the vacuole formation, nitroblue tetrazolium dye reduction, lysosomal enzyme activity, nitric oxide, myeloperoxidase in neutrophil and on neutrophil adherence assay. Results: The cool and hot water polysaccharides of Microporus vernicipes significantly improved the activities of macrophages and neutrophils as demonstrated by an elevated the formation of vacuole following by a high production of nitric oxide, lysosomal enzyme and superoxide. Those polysaccharides extracts of Microporous vernicipes also caused an increase in release of myeloperoxidase by neutrophils. Results also showed that neutrophils treated with polysaccharides extracts of Microporous vernicipes adhered more on a plastic surface than those untreated. It has been also found that polysaccharides of Microporous vernicipes may have cytotoxic effect at high concentration with IC50 of 2.28 and 8.15 mg/mL for PHW and PCW respectively. Conclusion: The result of this study indicates the immunostimulatory activity of polysaccharides of Microporus vernicipes by stimulating activities of macrophages and neutrophils.