Screening, Growth Characterization and Alkaline Phosphatase Potential of Pseudomonas plecoglossicida from Mangrove Soil
S. Sankaralingam, B. Harinathan, S. Palpperuma, D. Kathiresan, S. Rajendran, T. Sivakumar, T. Shankar, G. Prabakaran, N. Sivakumar
Microbiology Research Journal International · pp. 1–11 · Published 19 Nov 2016
10.9734/BMRJ/2016/28488Abstract
The study was carried out to isolate phosphate solubilizing bacteria from mangrove soil collected from the Manakudi estuary, Kanyakumari, Tamilnadu, India. Around 20 colonies were obtained in Zobell marine agar plates screened on Pikovskaya’s agar plate for determining their phosphate solubilizing ability. Out of these 20 isolates, one isolate exhibited better phosphate solubilizing ability. Identification of strain was carried out and confirmed by cultural, biochemical and 16S rDNA sequences. The potent isolate was identified as Pseudomonas plecoglossicida. Various factors were tested for pH, temperature, carbon and nitrogen sources, tricalcium phosphate influenced bacterial growth and phosphate activity in static and shaking conditions and thus characterized.
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