Synthesis, Characterization of Tetrazole Derivatives, and Evaluation of Bacterial and Fungal Activity

Abstract

The study involved the use of microwaves to react the prepared hydrazone with sodium azide to form various heterocyclic tetrazole-derived pentacyclics using THF as a solvent. In addition to measuring the prepared compounds' melting points, physical and spectroscopic techniques, including FT-IR spectroscopy, proton spectroscopy, and carbon nuclear magnetic resonance (1H-NMR) and (13C-NMR), were used to verify the synthesis' efficacy and accuracy. Two bacterial isolates were examined to see how the synthesized compounds affected their growth: Staphylococcus aureus, a Gram-positive (G+) bacterium, and E. coli, a Gram-negative (G-) bacterium. Control samples were antibiotics like amoxicillin, and several of the compounds that were made had strong inhibitory action against the bacterial species that were employed. It was also investigated how some of the produced chemicals affected the development of the fungus Candida albicans. Some of the synthesized compounds demonstrated good inhibitory efficacy against the utilized fungus when tested against control samples of medicines like fluconazole. The compound (SU7) showed the highest activity, reaching 30 mg/ml at its highest concentration against bacteria and fungi, reaching 30 mg/ml.