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Research Article Open access CC BY 4.0

Grain-borne Fungi on Farmer Saved Sorghum (Sorghum bicolor L.)

Alex Machio Kange, Sylvans Ochieng Ochola, Eric Mosota Rosana

Asian Research Journal of Agriculture · pp. 179–192 · Published 1 Mar 2025

10.9734/arja/2025/v18i1656

Abstract

Sorghum (Sorghum bicolor (L.) Moench) is an under-utilized cereal crop grown mainly in arid and semi-arid lands (ASALs) with various uses as human food, fodder, feed, fuel and industrial use but its yield and quality is influenced factors during and after production. The aim of this study was to determine the occurrence of grain borne fungi on farmer saved sorghum cultivars at pre-and post-harvest value chain. Using direct-plating technique, four fungi genera and 12 species were identified in sampled sorghum grains from farmers and experimental plots. The influence of moisture content (MC) is crucial in growth and development on grain surfaces. Prevalent fungi isolated and identified from the grains were Aspergillus spp., Fusarium spp., Penicillium spp. and Rhizopus spp. An analysis using high performance liquid chromatography (HPLC) revealed that 30% of the tested samples revealed B1 and B2 strains of aflatoxin. The existence of mould on grains showed mycotoxin strains which result in deterioration of sorghum grains thus food insecurity among the small holder farmers.

Sorghum sampling grain fungi aflatoxin

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