GC-MS Analysis of Chemical Constituents of Hydroalcoholic Leaf Extract of Cissampelos Pareira and Their Anti-Diabetic Activity

Abstract

Aim: The present work deals with the GC-MS-analysis of chemical constituents of hydroalcoholic extract of Cissampelos pareira leaves and thier anti-diabetic activity. Methods: GC-MS analysis of extract was performed using Shimadzu QP-2010 plus with thermal desorption system 20. Acute oral toxicity of extract was done using the Organization of Economic Co-operation and Development (OECD) guideline 423. Diabetes was induced by single dose of streptozotocin 65 mg/kg, i.p. to all the rats except in rats of control group. Following which oral glucose tolerance test was performed and the rats were divided into various experimental groups. Various treatments continued for 21 days. Parameters such as blood glucose level, body weight, liver enzymes, lipid profiles and oxidative markers were checked. Results: GC-MS analysis of the extract identified 25 compounds present in it. Based on acute oral toxicity study three doses of hydroalcoholic extract of Cissampelos pareira leaves viz 100, 200 and 400 mg/kg were selected for evaluation of anti-diabetic activity. The extracts at doses 200 and 400 mg/kg BW were able to reduce blood sugar level, liver enzymes, total cholesterol, total triglyceride, low density lipoprotein and Malondialdehyde; and enhance body weight, high density lipoprotein and Glutathione significantly when compared to rats of negative control group. The effect of extract at dose 400 mg/kg was comparable to standard Glibenclamide. Conclusion: Results conclude that the chemical constituents present in the hydroalcoholic extract of Cissampelos pareira contained some anti-diabetic compounds possessing strong anti-diabetic activity.