A Validated RP-HPLC-UV Method for Simultaneous Estimation of Ceftriaxone and Sulbactum in Rat Plasma
B. M. Gurupadayya, N. S. Disha
International Research Journal of Pure and Applied Chemistry · pp. 1–8 · Published 19 Dec 2014
10.9734/IRJPAC/2015/12177Abstract
A reverse phase-liquid chromatographic method with UV detection is developed for simultaneous estimation of ceftriaxone sodium and sulbactam sodium in rat plasma. A simple protein precipitation technique was employed for the extraction of drugs from blank plasma. Chromatographic separation of the two drugs was achieved on C18 column (250 mm X 4.6 mm, i.d, 5 μm) using a mobile phase consisting of 10mM potassium dihydrogen orthophosphate buffer (pH- 5) and acetonitrile (90:10% v/v). The developed liquid chromatographic method eluted both the drugs with a reasonable retention time, acceptable symmetric peak shape and good resolution. The developed bio-analytical method was linear, accurate, and precise over the concentration range of 20-150 μg mL-1 for ceftriaxone and 10-75 μg mL-1 for sulbactam. The developed method was successfully applied to monitor ceftriaxone and sulbactam sodium concentrations in rat plasma.
References (6)
- 1 Practical HPLC Method Development [DOI]
- 2 A Novel High Performance Liquid Chromatographic Method for Simultaneous Determination of Ceftriaxone and Sulbactam in Sulbactomax [DOI]
- 3 Spectrofluorimetric Protocol for Ceftriaxone in Commercial Formulation and Human Plasma After Condensation with Formaldehyde and Ethyl Acetoacetate [DOI]
- 4 Assays of Sulbactam in the Presence of Ampicillin [DOI]
- 5 [Determination of sulbactam in human serum using capillary isotachophoresis].
- 6 Determination of ceftriaxone in human plasma by HPLC
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